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Multifunctional antibody dilution liquid and preparation method thereof

An antibody diluent, multi-functional technology, applied in the field of immunization, can solve the problems of reducing non-specific binding, false positive test results, unsuitable for long-term storage, etc., to reduce non-specific reactions, improve antibody stability, and good blocking. with protective effect

Inactive Publication Date: 2018-12-07
SUZHOU YOUJUN ENVIRONMENTAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These precise detections are inseparable from the development of immunology. The detection of disease markers is mainly based on the antibody-antigen binding reaction. The specific reaction between the surface site of the antigen and the Fab of the antibody occurs, and the antigen does not necessarily have only one binding site. The non-specific site may combine with other miscellaneous proteins, resulting in false positives or false negatives in the test results. In this case, the corresponding protective agent can be added to the diluent to reduce the non-specific binding; in addition, the protein is not suitable for For long-term storage, if it needs to be preserved, it needs to be protected by a suitable diluent

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A multifunctional antibody diluent, composed of the following components in parts by weight: 2 parts of sodium caseinate, 0.3 parts of gentamicin, 57 parts of sodium bicarbonate, 45 parts of sodium citrate, 16 parts of citric acid, 12 parts of sulfanilamide, 15 parts of potassium chloride, 8 parts of sodium chloride, 5 parts of cystine, 15 parts of sucrose, 0.6 parts of sodium hydroxide, 0.3 parts of bovine serum albumin, 0.4 parts of goat IgG, Tween -40 0.4 parts, Proclin 300 0.3 parts, deionized water 300 parts.

[0018] A method for preparing a multifunctional antibody diluent, comprising the following steps:

[0019] Step 1: Add casein sodium into deionized water, stir at 40°C at a stirring speed of 180rpm for 6h, then filter through a 0.45μm filter membrane, add gentamicin to prepare solution A, store at 4°C until use;

[0020] Step 2, add sodium bicarbonate, sodium citrate, citric acid, sulfanilamide, potassium chloride, sodium chloride, cystine, sucrose, and so...

Embodiment 2

[0024] A multifunctional antibody diluent, which is composed of the following components in parts by weight: 2 parts of sodium caseinate, 0.4 parts of gentamicin, 57 parts of sodium bicarbonate, 50 parts of sodium citrate, 17 parts of citric acid, 12 parts of sulfanilamide, 12 parts of potassium chloride, 12 parts of sodium chloride, 5 parts of cystine, 15 parts of sucrose, 0.5 parts of sodium hydroxide, 0.3 parts of bovine serum albumin, 0.5 parts of goat IgG, Tween -40 0.6 parts, Proclin 300 0.3 parts, deionized water 350 parts.

[0025] A method for preparing a multifunctional antibody diluent, comprising the following steps:

[0026] Step 1: Add casein sodium into deionized water, stir at 38°C for 6 hours at a stirring speed of 180rpm, then filter through a 0.45μm filter membrane, add gentamicin to prepare solution A, store at 5°C until use;

[0027] Step 2, add sodium bicarbonate, sodium citrate, citric acid, sulfanilamide, potassium chloride, sodium chloride, cystine, ...

Embodiment 3

[0031] A multifunctional antibody diluent, composed of the following components in parts by weight: 1 part of sodium caseinate, 0.3 parts of gentamicin, 52 parts of sodium bicarbonate, 38 parts of sodium citrate, 12 parts of citric acid, 10 parts of sulfanilamide, 9 parts of potassium chloride, 9 parts of sodium chloride, 5 parts of cystine, 13 parts of sucrose, 0.5 parts of sodium hydroxide, 0.2 parts of bovine serum albumin, 0.5 parts of goat IgG, Tween -40 0.5 parts, Proclin 300 0.2 parts, deionized water 280 parts.

[0032] A method for preparing a multifunctional antibody diluent, comprising the following steps:

[0033] Step 1: Add casein sodium into deionized water, stir at 40°C at a stirring speed of 190rpm for 7 hours, then filter through a 0.45μm filter membrane, add gentamicin to prepare solution A, store at 6°C until use;

[0034] Step 2, sequentially add sodium bicarbonate, sodium citrate, citric acid, sulfanilamide, potassium chloride, sodium chloride, cystine,...

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Abstract

The invention discloses a multifunctional antibody dilution solution and a preparation method thereof, and the multifunctional antibody dilution solution comprises, by weight, 1-3 parts of casein sodium, 0.1-0.5 part of gentamicin, 38-67 parts of sodium bicarbonate, 33-55 parts of sodium citrate, 11-21 parts of citric acid, 9-15 parts of p-aminobenzenesulfonamide, 7-18 parts of potassium chloride,6-13 parts of sodium chloride, 2-8 parts of cystine, 11-19 parts of sucrose, 0.3-1 part of sodium hydroxide, 0.1-0.6 part of bovine serum albumin, 0.1-0.7 part of goat IgG, 0.3-0.7 part of Tween 40,0.1-0.5 part of Proclin 300 and 200-400 parts of deionized water. The multifunctional antibody dilution solution is prepared by the synergistic action of the components. The multifunctional antibody dilution solution is simple in preparation method, and can provide good blocking and protection for an antibody so as to reduce the non-specific reaction and improve the stability of the antibody.

Description

technical field [0001] The invention relates to the technical field of immunization, in particular to a multifunctional antibody diluent and a preparation method thereof. Background technique [0002] With the continuous development of human science and technology, it also drives the development of the biological industry. Among them, the development of immunology has brought about increasingly accurate detection of diseases, and even early detection of diseases, so as to achieve early detection and early treatment, and at the same time, it can monitor the body at any time Changes in markers in order to observe the therapeutic effect and improve the therapeutic effect. These precise detections are inseparable from the development of immunology. The detection of disease markers is mainly based on the antibody-antigen binding reaction. The specific reaction between the surface site of the antigen and the Fab of the antibody occurs, and the antigen does not necessarily have onl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531
CPCG01N33/531
Inventor 凌云凌天阳金可心夏戊君
Owner SUZHOU YOUJUN ENVIRONMENTAL SCI & TECH
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