Potato stem tip stripping quick propagation technique method

A technique for peeling off the shoot tip and potato is applied in the field of potato seedling cultivation, which can solve the problems of high price of tissue culture medium and increase in cost, and achieve the effects of high seedling survival rate, increased reproduction speed and reduced infection probability.

Pending Publication Date: 2018-12-11
ZUNYI HUAFU AGRI BIOLOGICAL SCI & TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the price of existing tissue culture medium is relatively high, which increases the cost

Method used

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  • Potato stem tip stripping quick propagation technique method

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Embodiment Construction

[0021] The technical solution of this patent will be further described in detail below in conjunction with specific embodiments.

[0022] A kind of potato stem tip stripping rapid propagation technology method, comprises the steps:

[0023] (1) Choose undamaged and five-worm-eaten potato stems, soak the potato cubes with 0.50-1mg / kg gibberellin for 20 minutes, and then place the potato stems on the sand bed in the greenhouse to grow buds; when the buds grow to 4- When it is 5cm, cut the upper stem tip section of 1.5-3cm, and disinfect after peeling off the leaves that are easy to see with tweezers after disinfection.

[0024] (2) Wrap the stripped stem tip with gauze, then rinse with tap water for 20 minutes, disinfect with 70-75% ethanol for 10-20s, disinfect with 1% sodium hypochlorite for 5-6min, and rinse with sterile water 3-5 times, and dry the surface moisture of the stem tip section with sterilized filter paper.

[0025] (3) Peel off the stem tip section after the ab...

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Abstract

The invention discloses a potato stem tip stripping quick propagation technique method. A seedling bed is prepared by mixing field soil, peat, vermiculite and perlite according to a mass ratio of 1:1:1:1; one stem joint is used in the sectioning propagation process, namely that one leaf is matched with one joint; the upper end is flush with the blade, the lower end is reserved with length of 1 to2cm, and the stem joint is slantwise inserted or flatly put at the surface of the culture medium. The potato stem tip stripping quick propagation method has the advantages that the culture medium is simplified and optimized, the propagation speed of potato is accelerated, the operation is simple, the virus carrying amount is fewer, and the survival rate of seedlings is high; by arranging the vermiculite and perlite, the infection probability of the seedlings is reduced.

Description

technical field [0001] The invention relates to the field of potato seedling cultivation, in particular to a method for rapid propagation of potato stem tip stripping. Background technique [0002] Potato, as an important grain, vegetable and industrial raw material, has the characteristics of short growth cycle, high yield per unit area, rich tuber nutrition and wide application, and has become the fourth largest crop after rice, wheat and corn in the world. large food crops. With the adjustment of the agricultural industrial structure, the planting area of ​​potatoes continues to expand. With the continuous advancement of agricultural technology, potato detoxification technology has also been widely used in the cultivation of potatoes. Potato virus-free technology is to cut potato shoots to produce cones, carry out tissue culture, cultivate virus-free test tube seedlings, and then produce virus-free tubers. Furthermore, the combination of stem tip virus-free, tissue cul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G22/25A01G24/10A01G24/15A01G24/28
CPCA01H4/008A01G22/25A01G24/10A01G24/15A01G24/28
Inventor 赵光平
Owner ZUNYI HUAFU AGRI BIOLOGICAL SCI & TECH CO LTD
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