A Molecular Marker of Maize Chromosome 9 Anthesis Qtl Locus and Its Application

Abstract

The invention relates to the field of biotechnology, and discloses a molecular marker of a QTL locus in the flowering stage of the No. 9 chromosome of maize and an application thereof. The molecular marker consists of two pairs of PCR primers JAAS4374 and JAAS4375. The present invention uses a BC1RIL population as a material, through a series of processes such as genomics analysis, molecular marker development, phenotype identification, QTL analysis, etc., to identify a maize flowering stage between the InDel markers JAAS4374 and JAAS4375 on the No. 9 chromosome of maize QTL loci. This locus can explain 10.4% of the phenotypic variation at the silking stage and 3.4% of the phenotypic variation at the pollination stage. The analysis showed that the marker can be used in the prediction and selection of maize flowering period.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a molecular marker of a QTL locus in the flowering stage of the No. 9 chromosome of maize and an application thereof. Background technique [0002] Corn is one of the three most important crops in the world, and it is also the main three-way crop of grain, economics and feed in my country. Before 2010, the sown area and total output both ranked second. In 2014, the sown area of ​​corn in my country reached 36.2 million hectares. Since then, it has become the largest food crop in my country and occupies a very important position in China's agricultural production and national economic development. [0003] In recent years, due to factors such as land transfer, large-scale operation, and urbanization construction, as well as the transfer of young and middle-aged laborers from rural areas to cities, corn production management has been extensive and backward, with low returns. Many rea...

AI Technical Summary

Problems solved by technology

Compared with Arabidopsis and rice, on the one hand, the maize genome is larger, with more repetitive sequences, and the genome structure is more complex; Susceptible to environmental influences, cloning is difficult

In addition, limited by the type of mapping population, the type of markers used, the accuracy of the genetic linkage map, and environmental control, there is no consistent result for the QTL analysis of maize flowering period.

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