A kind of preparation method of functionalized SERS platform and its detection application to ATP

A functional and platform technology, applied in the field of functionalized SERS platform preparation, can solve the problems of poor understanding of the biological basis of treatment, etc., to achieve the effect of improving sensitivity, strong SERS signal, and expanding the scope of application
CN109060764BActive Publication Date: 2020-12-29安徽中科赛飞尔科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
安徽中科赛飞尔科技有限公司
Publication Date
2020-12-29

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Abstract

The invention discloses a functionalized SERS (Surface-Enhanced Raman Scattering) platform preparation method and application in ATP detection thereof, and relates to the technical field of biologicaldetection. The functionalized SERS platform preparation method comprises the following steps of: step 1, preparing a gold nanoparticle solution; step 2, performing gold nanoparticle modification on acupuncture needles: immersing acupuncture needle bodies cleaned with ethanol in a 3-mercaptopropyltriethoxysilane ethanol solution of 10% (volume ratio) at 4 DEG C for 24 hours, and cleaning and drying the acupuncture needle bodies with ethanol after taking out to immerse in the gold nanoparticle solution; step 3, performing functionalization of Raman probes on acupuncture needles: immersing a acupuncture needle-like SERS substrate prepared in step 2 in a Raman probe solution at room temperature, taking out and drying the SERS substrate after treatment, and washing with pure water and storingat room temperature. According to the functionalized SERS platform preparation method and application in ATP detection thereof, gold nanoparticle modification and Raman probe functionalization are sequentially performed on the acupuncture needles, and the acupuncture needles are applied to ATP detection, so that the detection sensitivity is improved to obtain stronger SERS signals.
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Description

technical field

[0001] The invention belongs to the technical field of biological detection, in particular to a preparation method of a functionalized SERS platform and its application to ATP detection. Background technique

[0002] In traditional Chinese medicine, acupuncture has been used as complementary and alternative medicine for more than 2,000 years. Among acupuncture therapies, acupuncture is widely accepted worldwide and primarily used to treat acute and chronic pain, but little is known about the biological basis of its treatment. In a recent study, Goldman et al. reported the release of adenosine triphosphate (ATP) during acupuncture. After ATP is released, it acts as a transmitter that binds to purinergic receptors, and then rapidly degrades to adenosine to suppress pain. In addition to its antinociceptive effects, ATP also plays a key role in energy storage and signaling in biological systems. Most importantly, abnormal ATP levels in human body fluids are cl...

Claims

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