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A kind of preparation method of functionalized SERS platform and its detection application to ATP

A functional and platform technology, applied in the field of functionalized SERS platform preparation, can solve the problems of poor understanding of the biological basis of treatment, etc., to achieve the effect of improving sensitivity, strong SERS signal, and expanding the scope of application

Active Publication Date: 2020-12-29
安徽中科赛飞尔科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In acupuncture therapy, acupuncture is widely accepted worldwide and used primarily for the treatment of acute and chronic pain, but little is known about the biological basis of its treatment

Method used

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  • A kind of preparation method of functionalized SERS platform and its detection application to ATP
  • A kind of preparation method of functionalized SERS platform and its detection application to ATP
  • A kind of preparation method of functionalized SERS platform and its detection application to ATP

Examples

Experimental program
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Effect test

Embodiment 1

[0047] A method for preparing a functionalized SERS platform, comprising the steps of:

[0048] Step 1, preparation of gold nanoparticle solution;

[0049] Synthesis of gold nanoparticles by reducing chloroauric acid with trisodium citrate;

[0050] Step 2. Gold nanoparticles modified acupuncture needles:

[0051] (1) Clean the acupuncture needles with ethanol. After cleaning, immerse the acupuncture needle body in a 10% (volume ratio) 3-mercaptopropyltriethoxysilane ethanol solution at 4°C for 24 hours;

[0052] (2), the acupuncture needles obtained in (1) are washed 3 times with ethanol and dried under nitrogen flow;

[0053] (3), immersing the washed and dried acupuncture needle body obtained in (2) into the gold nanoparticle solution obtained in step 1;

[0054] Step 3. Functionalization of Raman probes to acupuncture needles:

[0055] Soak the acupuncture needle-like SERS substrate prepared in step 2 in a concentration of 5 × 10 -5 mol / L Raman probe solution for 2 ho...

Embodiment 2

[0073] Acupuncture needle detection probe MA modified with Au NPs

[0074] Prepare 7 series of MA solutions with concentrations ranging from 100umol / L to 1umol / L, take the 7 acupuncture needles modified with Au NPs in Example 1 and soak them in the solutions of 7 concentrations for 5min, take them out and dry them, and perform a Raman test. Test under the spectrometer, the obtained spectrogram.

[0075] 7 series concentrations are 1×10 -4 mol / L, 7.5×10 -5 mol / L, 5×10 -5 mol / L, 1×10 -5 mol / L, 7.5×10 -6 mol / L, 5×10 -6 mol / L, 1×10 -6 mol / L;

[0076] Such as Figure 4 As shown, active needles showed high sensitivity and stable detection for MA detection.

Embodiment 3

[0078] Au NPs and MA functionalized acupuncture needles for detection of ATP

[0079] Prepare 7 series of ATP solutions with concentrations ranging from 25nM to 1000nM, take the 7 acupuncture needles modified with Au NPs and MA in Example 1, soak them in the solutions of 7 concentrations for 5min, take them out and dry them, and put them under the Raman spectrometer Test, get the spectrogram.

[0080] The concentrations of the 7 series are 2.5×10 -8 mol / L, 5.0×10 -8 mol / L, 7.5×10 -8 mol / L, 1.0×10 - 7 mol / L, 2.5×10 -7 mol / L, 5.0×10 -7 mol / L, 7.5×10 -7 mol / L, 1×10 -6 mol / L;

[0081] Such as Figure 5 As shown, by using electrostatic adsorption and Au-N bonds, the probe MA molecule modified on the SERS active needle exhibits high SERS signal; the addition of ATP can effectively inhibit the SERS signal of MA; the affinity between MA and ATP is higher than that of MA The affinity with the substrate is strong, so the addition of ATP keeps MA away from the substrate with t...

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Abstract

The invention discloses a functionalized SERS (Surface-Enhanced Raman Scattering) platform preparation method and application in ATP detection thereof, and relates to the technical field of biologicaldetection. The functionalized SERS platform preparation method comprises the following steps of: step 1, preparing a gold nanoparticle solution; step 2, performing gold nanoparticle modification on acupuncture needles: immersing acupuncture needle bodies cleaned with ethanol in a 3-mercaptopropyltriethoxysilane ethanol solution of 10% (volume ratio) at 4 DEG C for 24 hours, and cleaning and drying the acupuncture needle bodies with ethanol after taking out to immerse in the gold nanoparticle solution; step 3, performing functionalization of Raman probes on acupuncture needles: immersing a acupuncture needle-like SERS substrate prepared in step 2 in a Raman probe solution at room temperature, taking out and drying the SERS substrate after treatment, and washing with pure water and storingat room temperature. According to the functionalized SERS platform preparation method and application in ATP detection thereof, gold nanoparticle modification and Raman probe functionalization are sequentially performed on the acupuncture needles, and the acupuncture needles are applied to ATP detection, so that the detection sensitivity is improved to obtain stronger SERS signals.

Description

technical field [0001] The invention belongs to the technical field of biological detection, in particular to a preparation method of a functionalized SERS platform and its application to ATP detection. Background technique [0002] In traditional Chinese medicine, acupuncture has been used as complementary and alternative medicine for more than 2,000 years. Among acupuncture therapies, acupuncture is widely accepted worldwide and primarily used to treat acute and chronic pain, but little is known about the biological basis of its treatment. In a recent study, Goldman et al. reported the release of adenosine triphosphate (ATP) during acupuncture. After ATP is released, it acts as a transmitter that binds to purinergic receptors, and then rapidly degrades to adenosine to suppress pain. In addition to its antinociceptive effects, ATP also plays a key role in energy storage and signaling in biological systems. Most importantly, abnormal ATP levels in human body fluids are cl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 杨良保李盼毛妹于建强唐祥虎
Owner 安徽中科赛飞尔科技有限公司
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