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Kit for detecting anti-depressant drugs in serum and plasma by liquid chromatography tandem mass spectrometry and application thereof

A technology of tandem mass spectrometry and liquid chromatography, which is applied in the field of kits for detection of antidepressant drugs by liquid chromatography tandem mass spectrometry, to achieve the effect of reducing the matrix effect

Inactive Publication Date: 2018-12-25
HANGZHOU BAICHEN MEDICAL INSTR CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, a public document published on the market with an announcement date of August 2014 discloses "Simultaneous determination of 9 antidepressants in Chinese patent medicines and health products by ultra-high performance liquid chromatography-tandem mass spectrometry". - The method of tandem mass spectrometry completes the detection of antidepressant drugs, but its applicable objects are Chinese patent medicines and health care products. Whether antidepressant drugs are contained in the drugs taken is not directly related to the actual antidepressant drugs in the body of the user

Method used

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  • Kit for detecting anti-depressant drugs in serum and plasma by liquid chromatography tandem mass spectrometry and application thereof
  • Kit for detecting anti-depressant drugs in serum and plasma by liquid chromatography tandem mass spectrometry and application thereof
  • Kit for detecting anti-depressant drugs in serum and plasma by liquid chromatography tandem mass spectrometry and application thereof

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Embodiment 1

[0042] 1. Materials:

[0043] 1.1 Instruments and reagents:

[0044] Instruments: 10mL measuring flask, volumetric flask, vortex analyzer, centrifuge, liquid chromatography system, triple quadrupole mass spectrometer.

[0045] Reagent: kit, test serum and / or test plasma.

[0046] 2. Reagent preparation steps:

[0047] 2.1 Preparation of drug standard solution:

[0048] Take 10 mg of the drug standard substance, place it in a 10 mL volumetric bottle, dissolve it with the drug extraction composition, dilute to the volume, and obtain a 1.0 mg / mL drug standard solution.

[0049] Dissolved with 50% methanol aqueous solution, constant volume, and diluted to make standard solutions with different concentrations. 100ng / mL, 250ng / mL, 500ng / mL, 1000ng / mL standard solutions.

[0050] 2.2 Preparation of drug internal standard solution:

[0051] Take the drug internal standard compound, use the drug extraction composition to dissolve the drug internal standard compound, and transfer ...

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Abstract

The invention provides a kit for detecting anti-depressant drugs in serum and plasma by liquid chromatography tandem mass spectrometry. The kit comprises drug standard substances, drug internal standardization compounds, drug extraction compositions, negative plasma and a diluent. The drug standard substances comprise amfebutamone, oxybupropion, citalopram, Escitalopram, venlafaxine, O-desmethylvenlafaxine, duloxetine, fluoxetine, norfloxetine, fluvoxamine, mirtazapine, paroxetine, sertraline and trazodone. The drug internal standardization compounds comprise amfebutamone-d9, oxybupropion-d6,citalopram-d6, venlafaxine-d6, O-desmethylvenlafaxine-d6, duloxetine-d3, fluoxetine-d6, norfloxetine-d6, fluvoxamine-d4, mirtazapine-d3, paroxetine-d6, sertraline-d3 and trazodone-d6. The drug extraction compositions comprise, by volume, 60% of methanol solution, 20% of acetonitrile solution, 10% of isopropyl alcohol solution and 10% of purified water. The diluent comprises 50 % of methanol waterfluid. The kit can be used for simultaneous detection of the anti-depressant drugs and active metabolites, the detection time is short, and flux is high.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a liquid chromatography tandem mass spectrometry kit for detecting antidepressant drugs and its application. The kit is suitable for using tandem mass spectrometry to detect antidepressant drugs and their metabolites. Background technique [0002] Antidepressive drugs refer to a group of psychotropic drugs mainly used to treat mental illness with depression as the prominent symptom. There are two common types of antidepressant drugs: monoamine oxidase inhibitors (MAOIs) and tricyclic antidepressants. (TCA) and tetracyclic antidepressants. However, improper use of antidepressants can cause side effects such as drowsiness, dry mouth, blurred vision, constipation, rapid heartbeat, difficulty urinating, and postural hypotension, and may also cause serious cardiovascular side effects, urinary retention, and intestinal paralysis. , severe cases even directly lead to acute poisoning o...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 彭军吴超超高强许洪涛
Owner HANGZHOU BAICHEN MEDICAL INSTR CO LTD
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