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Method for determining content of medicine made from snakegourd root or snakegourd root-containing material

A method of determination, trichosanthin technology, applied in the direction of measuring devices, material separation, analysis of materials, etc., can solve the problems of complex sample processing process, and achieve the effect of short detection time, high repeatability and simple mobile phase

Active Publication Date: 2019-01-11
SICHUAN NEO GREEN PHARMA TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are many amino acid components in Trichosanthum pollen, and the existing detection methods include citrulline and γ-aminobutyric acid (Liu Wen, Determination of citrulline and γ-aminobutyric acid in Trichosanthes pollen), using pre-column derivatization reversed-phase High-efficiency chromatographic peaks are detected, and the sample processing process is more complicated

Method used

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  • Method for determining content of medicine made from snakegourd root or snakegourd root-containing material
  • Method for determining content of medicine made from snakegourd root or snakegourd root-containing material
  • Method for determining content of medicine made from snakegourd root or snakegourd root-containing material

Examples

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Effect test

Embodiment 1

[0060] Example 1 Method for determination of trichosanthes medicinal material content of the present invention

[0061] Chromatographic conditions and system suitability test: phenyl-hexyl silane bonded silica as filler (column length is 250mm, inner diameter is 4.6mm, particle size is 5μm); acetonitrile-water (4:96) is used as mobile phase; detection wavelength It is 224nm. The number of theoretical plates should not be less than 3000 calculated based on the tryptophan peak.

[0062] Preparation of the reference substance solution Take an appropriate amount of the tryptophan reference substance, accurately weigh it, and add dilute ethanol to make a solution containing 10 μg per 1 ml.

[0063] Preparation of the test solution: Take about 0.5g of the powder (passed through a No. 3 sieve), accurately weigh it, place it in a stoppered conical flask, add 25ml of dilute ethanol, weigh it, ultrasonically treat it for 20 minutes, and let it cool. Weigh the weight again, make up the lost w...

Embodiment 2

[0065] Example 2 Investigation on the Methodology of the Determination of Trichosanthes medicinal Material Content of the Invention

[0066] 1 instrument

[0067] Agilent 1200 high performance liquid chromatograph, Shimadzu 20AD high performance liquid chromatograph, Shimadzu 20AT high performance liquid chromatograph;

[0068] Electronic balance: ME204E / 02, MS205DU, XP26 (Mettler-Toledo Instruments Co., Ltd.);

[0069] Ultrapure water machine: Cell type 1810A (Shanghai Mole Scientific Instrument Co., Ltd.);

[0070] Ultrasonic cleaner: KQ5200DB type (600W, 40KHz; Kunshan Ultrasonic Instrument Co., Ltd.);

[0071] Column: Agilent Pheny-Hexyl 4.6×250mm 5μm, DiKMA Pheny-Hexyl4.6×250mm5μm, Philomon Pheny-Hexyl 4.6×250mm 5μm

[0072] 2 reagents and medicinal materials

[0073] Tryptophan reference substance (China Institute for the Control of Pharmaceutical and Biological Products, batch number: 140686-201303, content is 99.9%).

[0074] Acetonitrile (Sigma, chromatographically pure); water is ...

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Abstract

The invention provides a method for determining the content of a medicine made from snakegourd root or a snakegourd root-containing material. The method adopts HPLC (High Performance Liquid Chromatography) and comprises the following operation steps: a, preparing a solution of a to-be-inspected sample: adding dilute ethanol to the to-be-inspected sample, carrying out ultrasonic treatment for 20-40minutes, standing to cool, weighing, complementing the lost weight by using the dilute ethanol, uniformly shaking, filtering and taking the filtrate to obtain the solution of the to-be-inspected sample; b, preparing a reference liquor: accurately weighing appropriate amount of tryptophan reference substance and adding a dilute ethanol solution to obtain the reference solution; and c, respectivelyaccurately injecting the reference solution and the solution of the to-be-inspected sample into a liquid chromatograph and eluting by taking acetonitrile and water as mobile phases, wherein the chromatographic conditions are that: phenyl-hexyl silane bonded silica gel is used as a filler; the acetonitrile and the water at the ratio of (4-10):(90-96) are used as the mobile phases; the flow rate is0.8-1.2ml / min; the column temperature is 25-35 DEG C; the detection wavelength is 224nm; and the number of theoretical plates is not less than 3000 according to the peak of the tryptophan. The methodprovided by the invention can be used for detecting the content of tryptophan in the snakegourd root and has the advantages of being strong in operability, simple in sample processing process, simplein the mobile phases, high in repeatability and short in detection time.

Description

Technical field [0001] The invention relates to a method for determining the content of trichosanthes or a medicine prepared with trichosanthes as a raw material. Background technique [0002] Trichosanthes is a traditional Chinese medicine with a long history and a wide range of uses. Trichosanthin has the effects of inducing labor and anti-tumor. Trichosanthes kirilowii Maxim. Trichosanthes kirilowii Maxim. or Trichosanthes Uniflora Hao, a trichosanthes uniflora Hao, is mainly produced in Henan, Shandong, Jiangsu, Guizhou. Anhui and other places. At present, there are many original plants of trichosanthes in all parts of the country. The curative effects of trichosanthes from different plant sources are not exactly the same, and some counterfeit products have certain adverse reactions. [0003] There are many detection methods reported for Trichosanthes, such as Lu Jianjiang, Extraction and Content Determination of Trichosanthes Polysaccharides, Tianjin Pharmaceuticals, Volume...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/02
CPCG01N30/02G01N30/06G01N2030/027
Inventor 周厚成胡昌江张玉婷戴德蓉李文兵周维冯健
Owner SICHUAN NEO GREEN PHARMA TECH DEV
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