Convenient and stable detection kit for homocysteine and detection method

Abstract

The invention provides a convenient and stable detection kit for homocysteine and a detection method. The kit comprises a reagent R1, a reagent R2, and a HCY liquid calibration solution. The reagent R1 contains a buffer, a reducing agent, a serine, a coenzyme preservative, and a surfactant. The reagent R2 contains a buffer, a surfactant, a lactate dehydrogenase, a stabilizer, an antioxidant, a preservative, a cystathionine beta-synthase, and a cystathionine beta-lyase. The HCY liquid calibration solution contains a stabilizer, a preservative, and homocysteine. The antioxidant is added into thekit, so that the attenuation of the enzyme under various interference factors is inhibited, and the stability of the kit in the special environment and the validity period is effectively improved.

Description

technical field [0001] The invention belongs to the field of medical in vitro diagnostic reagents, and in particular relates to a convenient and stable homocysteine ​​detection kit and detection method. Background technique [0002] Blood homocysteine ​​(HCY) is a risk factor for stroke and other cardiovascular and cerebrovascular diseases. Its level is significantly increased, and it can be diagnosed as "hyperhomocysteinemia", especially if accompanied by a history of hypertension. Many scholars believe that elevated blood homocysteine ​​has become an independent risk factor for atherosclerosis, and can cause a variety of diseases, because it can affect the synthesis and function of reduced glutathione, and affect normal liver function. The specificity and sensitivity of this indicator for the diagnosis of chronic liver disease are better than those of conventional detection; another new concept is that essential hypertension accompanied by elevated plasma HCY (>10umol / L...

AI Technical Summary

Problems solved by technology

Although the sensitivity and specificity are good, the instrument is expensive and cannot be widely used

High-performance liquid chromatography (HPLC) is a relatively mature and widely used method at present. The disadvantage is that there are many variations in sample processing, chromatographic conditions, sample detection and quantification, making it difficult to standardize

However, the attenuation of cystathionine β-lyase in the above two kits is still relatively fast, resulting in poor stability of the kits for cystathionine cycle enzyme method under the expiration date.

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