A green extraction, enrichment, separation and purification method of neophytadiene in tobacco raw materials

Abstract

A method for the green extraction, enrichment, separation and purification of neophytadiene in tobacco raw materials, which is to add tobacco leaf powder into pure water, and then use a microwave solvent-free extraction process, and control the conditions of high power and low power and corresponding extraction time to extract To enrich neophytadiene, the neophytadiene-rich tobacco essential oil extracted by microwave solvent-free extraction is further separated by column chromatography, using silica gel as the stationary phase, and using a mixed solution of petroleum ether and ethyl acetate in different proportions As an eluent, neophytadiene with a content of 70% to 95% is eluted from low polarity to high polarity gradient. The method of the invention is environmentally friendly, fast and efficient, has good enrichment effect, and can further separate and purify the essential oil enriched with neophytadiene.

Description

technical field [0001] The invention relates to the technical field of enrichment, separation and purification of neophytadiene, an important endogenous tobacco aroma substance, and the isolated neophytadiene is used as tobacco essence to enrich the original aroma of tobacco, soften smoke, and improve sweetness , Cover up miscellaneous gas, improve the quality of tobacco products. Background technique [0002] Under the industry background of tobacco harm reduction and tar reduction, increasing cigarette flavor and maintaining fragrance has become one of the research focuses of cigarette enterprises. Tobacco endogenous aroma substances are the aroma components or precursors of tobacco itself. Adding characteristic tobacco endogenous aroma substances to shredded tobacco can enhance the flavor and concentration, improve the taste, and significantly improve the smoking quality of cigarettes. . Tobacco terpenoids are an important class of tobacco aroma components, and neophyta...

AI Technical Summary

Problems solved by technology

Literature (Kang Ming'e et al., Synthesis of Neophytadiene [J]. Perfume, Fragrance and Cosmetics, 2016, 4, 1-3) proposed to use phytol as raw material to obtain neophytadiene through hydrogenation reduction reaction, but the synthetic product is safe to use Relatively low

Extracting neophytadiene from natural tobacco leaves also has related reports, literature (R.L.Rowland, Flue-cured Tobacco.11.Neophytadiene[J].J.Am.Chem.Soc., 1957,79,5007-1010) and literature ( R.N.Gladding, et al.Identification of Neophytadiene in Burley Tobacco and in Cigarette Smoke[J].J.Org.Chem.,1959,24,1358-1359) have proposed to use alkanes as extraction solvents to extract from tobacco leaves by Soxhlet reflux After the fat-soluble substances are separated, neophytadiene is separated by chromatographic separation, recrystallization, etc., but this method uses the traditional reflux extraction method, the extraction solvent is an organic solvent, and there are many other fat-soluble impurities extracted, making separation difficult , heavy workload

Literature (Deng Xiaohua, Enrichment and Application of Neophytadiene in Tobacco Leaves [J]. Food Industry, 2009, 2, 36-38) used supercritical extraction technology and molecular distillation technology to extract and separate tobacco leaves, and obtained phytodiene rich in phytodiene Tobacco extracts of dienes, but neophytadiene monomers were not isolated