Method for preparing total flavonoids of herba Euphorbiae Flavescentis

A technology for catching yellow grass and total flavonoids is applied in the field of preparation of total flavonoids from yellow grass, can solve problems such as separation and purification rarely involved, and achieve the effect of simple preparation method

Inactive Publication Date: 2019-01-25
SOUTHWEST MEDICAL UNIVERISTY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the research on the total flavonoids of C. chinensis mainly focuses on the extraction and pharmacological effects of the total flavonoids of C. chinensis, but seldom involves the research on separation and purification.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A preparation method of total flavonoids of C. chinensis, the steps are as follows:

[0017] (1) Rough extraction: take an appropriate amount of C. chinensis powder through a No. 4 sieve, add 10 times the amount of 55% ethanol solution, heat and reflux extraction for 3 times, each time for 1.0h, filter, combine the filtrates, concentrate under reduced pressure, dry, and get rid of Crude extract of total flavonoids from Huangcao.

[0018] (2) Refining: the crude extract of Herba japonica produced in step (1) is made into a solution of 5.0 mg / ml with distilled water, and a certain volume of Herba japonica crude extract solution is measured, and a certain volume of 60 Among ~90 mesh polyamides, the ratio of the mass (mg) of the crude extract of C. japonica to the volume (ml) of the polyamide is 22.5 mg / ml. After static adsorption in a constant temperature oscillator for 2 hours, filter, pour the filtered polyamide into a chromatographic column, first wash with water, then...

Embodiment 2

[0021] A preparation method of total flavonoids of C. chinensis, the steps are as follows:

[0022] (1) Rough extraction: Take an appropriate amount of C. chinensis powder through a No. 4 sieve, add 8 times the amount of 75% ethanol solution, heat and reflux for extraction twice, each time for 1.5h, filter, combine the filtrates, concentrate under reduced pressure, dry, and get rid of Crude extract of total flavonoids from Huangcao.

[0023] (2) Refining: the crude extract of Herba japonica produced in step (1) is made into a solution of 5.0 mg / ml with distilled water, and a certain volume of Herba japonica crude extract solution is measured, and a certain volume of 60 Among ~90 mesh polyamides, the ratio of the crude extract mass (mg) to the polyamide volume (ml) is 20.0 mg / ml. After static adsorption in a constant temperature oscillator for 3 hours, filter, pour the filtered polyamide into a chromatographic column, first wash with water, then elute with 15% ethanol, and fin...

Embodiment 3

[0026] A preparation method of total flavonoids of C. chinensis, the steps are as follows:

[0027] (1) Rough extraction: Take an appropriate amount of C. chinensis powder through a No. 4 sieve, add 12 times the amount of 55% ethanol solution, heat and reflux extraction for 3 times, each time for 1.0h, filter, combine the filtrates, concentrate under reduced pressure, dry, and get rid of Crude extract of total flavonoids from Huangcao.

[0028] (2) Refining: Mix the crude extract of total flavonoids of C. chinensis in 2 times the amount of 60-90 mesh polyamide, dry under reduced pressure, put the sample on a 60-90 mesh polyamide chromatography column by dry method, and load the sample The amount is 21.5 mg crude extract / ml polyamide. Wash with water first, then elute with 15% ethanol, and finally elute with 75% ethanol with pH=7, the elution speed is 1.5ml / min. Collect the eluted part with 75% ethanol, concentrate under reduced pressure, and dry to obtain the total flavonoid...

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Abstract

A method for preparing total flavonoids of herba Euphorbiae Flavescentis belong to the field of effective parts of traditional Chinese medicine. The preparation method comprises the following steps: (1) roughly extracting the herba Euphorbiae Crataegi with 55%-75% ethanol was extract by heating and reflux; 2, refine: adopting polyamide column chromatography, elute with water, 15% ethanol and 75% ethanol in turn, collecting 75% eluent, concentrating unde reduced pressure, and drying to obtain total flavonoids of herba Euphorbiae Humifusae; (3) determination of total flavonoids content: the content of total flavonoids was determined by UV spectrophotometry. The content of total flavonoids was more than 50% according to arborvitae. The content of total flavonoids of herba cynanchi paniculatimeets the requirements of the effective part preparation of traditional Chinese medicine, and can be used as the raw material medicine of the traditional Chinese medicine preparation.

Description

1. Technical field [0001] The invention belongs to the field of effective parts of traditional Chinese medicines, and relates to a preparation method of total flavonoids of C. chinensis. 2. Background technology [0002] Huangcao is the dry aboveground part of Penthorum chinense pursh. It is a folk herbal medicine of the Miao nationality. It is mainly produced in Sichuan, Guizhou, Hunan and other places. It is a local medicinal material in Gulin, Luzhou. Catch yellow grass, also known as pulling root vegetables, water Zelan, water willow, and mountain eel. Herba Rhizome is flat in nature, bitter in the mouth, slightly pungent, nontoxic, and returns to the Liver and Kidney meridians. It has the effects of clearing away heat and detoxification, reducing yellowing and dampness, promoting blood circulation and dispelling blood stasis, diuresis and detumescence, and is widely used in the treatment of various types of hepatitis, cholecystitis, fatty liver, etc. [0003] Chamomil...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61P39/06A61P1/16A61P39/00
CPCA61P1/16A61P39/00A61P39/06A61K36/185A61K2236/55A61K2236/39A61K2236/51A61K2236/333
Inventor 袁叶飞白雪
Owner SOUTHWEST MEDICAL UNIVERISTY
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