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Kit for detecting abundance of common probiotics

A technology of probiotics and kits, which is applied in the field of kits for detecting the abundance of common probiotics, can solve problems such as complex composition of intestinal flora, low amplification efficiency, lack of primers, etc., to shorten the detection cycle and reduce detection costs , the effect of high amplification efficiency

Active Publication Date: 2019-01-25
GENETALKS BIO TECH CHANGSHA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The composition of the intestinal flora is complex, and many strains are extremely close to each other. Directly using fluorescent PCR to amplify the intestinal flora often has poor specificity and low amplification efficiency. There are no primers that can cope with such complex samples in the prior art

Method used

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  • Kit for detecting abundance of common probiotics
  • Kit for detecting abundance of common probiotics
  • Kit for detecting abundance of common probiotics

Examples

Experimental program
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Embodiment 1

[0059] Primer sequence:

[0060] Faecalibacterium:

[0061] Upstream sequence: GGTCTTCGGATTGTAAACTCCTG

[0062] Downstream sequence: GTAATTCCGGACAACGCTTGTG

[0063] lactobacillus:

[0064] Upstream sequence: GTGGAACTCCATGTGTAGCGG

[0065] Downstream sequence: GGCGGAAACCCTCCAACAC

[0066] Bifidobacterium:

[0067] Upstream sequence: CGGGTGAGTAATGCGTGACC

[0068] Downstream sequence: TGATAGGACGCGACCCCA

[0069] Akkermansia muciniphila:

[0070] Upstream sequence: CGGCACATGATACTGCGAGAC

[0071] Downstream sequence: TCAGTTAATGTCCAGGAACCCG

[0072] Total bacteria:

[0073] Upstream sequence: CCTACGGGAGGCAGCAG

[0074] Downstream sequence: ATTACCGCGGCTGCTGG.

[0075] The composition of the qPCR reaction system is:

[0076] Element

Volume (μL)

Fluorescent dyes

5

Forward primer (2.5 μM)

0.5

Reverse primer (2.5 μM)

0.5

Template DNA (2ng / μL)

1

UDG enzyme

0.01

water

Make up to 10 μL

[0077] The flu...

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Abstract

The invention discloses a kit for detecting the abundance of common probiotics, as a result of their own technology, analyzing and designing from a large amount of data, the primers with the best specificity and amplification efficiency were screened out, including Faecalibacterium, lactobacillus, Bifidobacterium, akkermansia muciniphil and total bacterial prim that primers of the invention have good amplification specificity and high amplification efficiency, and can specifically amplify the correspond probiotics from the total DNA of the complex intestinal microbial flora, and the result isthe same as that of the mature method. The reagent kit of the invention can realize the rapid and intuitive detection of common probiotics, shortens the detection period and reduces the detection cost.

Description

technical field [0001] The invention relates to a kit for detecting the abundance of common probiotics. Background technique [0002] The intestinal flora includes approximately 2,000 species of bacteria. The composition of intestinal flora is closely related to living habits, diet and host genes, and there are large differences among individuals. Firmicutes, Bacteroidetes, and Actinomycetes, these three phyla dominate the human intestinal flora, anaerobic bacteria such as Bacteroides, Eubacterium, Bifidobacterium, Clostridium, Peptostreptococcus, etc. accounted for the main part of the intestinal flora, while facultative anaerobic bacteria such as Lactobacillus, Enterococcus, Streptococcus, Enterobacter, etc. only accounted for a small part. [0003] The intestinal flora and the host form a dynamic and balanced micro-ecosystem, and participate in various physiological activities: participate in the digestion and absorption of nutrients, ferment cellulose, oligosaccharides...

Claims

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Application Information

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IPC IPC(8): C12Q1/689C12Q1/6851C12Q1/06C12R1/225C12R1/01
CPCC12Q1/6851C12Q1/689C12Q2531/113C12Q2563/107
Inventor 宋卓王永利卢秦
Owner GENETALKS BIO TECH CHANGSHA CO LTD
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