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Method for purifying anthocyanin of black kidney bean peel through simulated moving bed chromatography

A technology for simulating mobile chromatography and anthocyanins, which is applied in the field of purifying black kidney bean skin anthocyanins by simulated mobile chromatography, can solve the problem of inability to efficiently and continuously purify black kidney bean skin anthocyanins, etc., and achieves increasing resin utilization, reducing concentration costs, improving The effect of production efficiency

Active Publication Date: 2019-02-15
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a method for purifying black kidney bean skin anthocyanins by simulated mobile chromatography, which is used to solve the problem of inability to efficiently and continuously purify the color of black kidney bean skin in the prior art Glycoside conundrum

Method used

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  • Method for purifying anthocyanin of black kidney bean peel through simulated moving bed chromatography

Examples

Experimental program
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Effect test

Embodiment 1

[0020] This method of purifying black kidney bean peel anthocyanins by simulated mobile chromatography:

[0021] The anthocyanin extract was prepared from black kidney bean husk. After pretreatment by concentration and filtration, the concentration of the anthocyanin extract was adjusted to 200 mg / mL, and then sent to simulated mobile chromatography.

[0022] Simulated mobile chromatography to purify anthocyanins: the anthocyanin extract obtained above is purified by the simulated mobile chromatography. The simulated mobile chromatography includes 20 chromatographic columns and is divided into an adsorption zone, a purification zone, a desorption zone, a regeneration zone and a water wash. The number of chromatographic columns in the five zones is 6, 4, 3, 4, and 3, respectively. Each zone is connected in series, with 1 feed port and 1 discharge port. The feed ports of the adsorption zone, refining zone, desorption zone and regeneration zone are the first chromatographic colum...

Embodiment 2

[0026] This method of purifying black kidney bean peel anthocyanins by simulated mobile chromatography:

[0027] The anthocyanin extract was prepared from black kidney bean husk. After pretreatment by concentration and filtration, the concentration of the anthocyanin extract was adjusted to 100 mg / mL, and then sent to simulated mobile chromatography.

[0028] Simulated mobile chromatography purification of anthocyanins: the anthocyanin extract obtained above is purified by the simulated mobile chromatography, and the simulated mobile chromatography is the same as in Example 1, wherein the adsorption zone, the purification zone, the desorption zone, the regeneration zone and the washing zone are processed. The feed liquids were 100 mg / mL anthocyanin extract after pretreatment, 15% ethanol aqueous solution, 45% ethanol aqueous solution, 100% ethanol aqueous solution and deionized water; adsorption zone, refining zone, desorption zone, regeneration zone and water washing zone. Th...

Embodiment 3

[0032] This method of purifying black kidney bean peel anthocyanins by simulated mobile chromatography:

[0033] The anthocyanin extract was prepared from black kidney bean husk. After pretreatment by concentration and filtration, the concentration of the anthocyanin extract was adjusted to 300 mg / mL, and then sent to simulated mobile chromatography.

[0034] Simulated mobile chromatography purification of anthocyanins: the anthocyanin extract obtained above is purified by the simulated mobile chromatography, and the simulated mobile chromatography is the same as in Example 1, wherein the adsorption zone, the purification zone, the desorption zone, the regeneration zone and the washing zone are processed. The feed liquids were 300 mg / mL anthocyanin extract after pretreatment, 20% ethanol aqueous solution, 40% ethanol aqueous solution, 80% ethanol aqueous solution and deionized water; adsorption zone, refining zone, desorption zone, regeneration zone and water washing zone. The f...

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Abstract

The invention relates to a method for purifying anthocyanin of black kidney bean peel through simulated moving bed chromatography. The method comprises the following steps: preparing an anthocyanin extract from the black kidney bean peel, pretreating, then adjusting the concentration of the anthocyanin extract to 100-300mg / mL, and conveying into simulated moving bed chromatography equipment; purifying the pretreated anthocyanin extract through the simulated moving bed chromatography, wherein simulated moving bed chromatography equipment consists of 20 chromatographic columns and is divided into an adsorption zone, a refining zone, a desorption zone, a regeneration zone and a water washing zone, the numbers of the chromatographic columns in the five zones are 6, 4, 3, 4 and 3 respectively,and the zones are serially connected; all the zones work at the same time during operation, each chromatographic column moves by one column position clockwise after the operating time reaches the switching time, and continuous operation is achieved by operating in such a way; the obtained purified anthocyanin is further concentrated in vacuum and freeze-dried to obtain refined anthocyanin. By themethod, continuous production can be achieved and the production efficiency is improved.

Description

1. Technical field: [0001] The invention relates to a separation and purification technology of simulated mobile chromatography in the field of food industry, in particular to a method for purifying black kidney bean peel anthocyanins by simulated mobile chromatography. 2. Background technology: [0002] Black kidney bean has a long history of cultivation and consumption in my country. Traditional Chinese medicine believes that it has the functions of promoting blood circulation and invigorating water, anti-aging and nourishing blood, nourishing blood and soothing the nerves, nourishing kidney and nourishing yin, nourishing liver and detoxification. It has physiological functions such as antioxidant, anti-tumor, and repair of pancreatic islet tissue damage, and has broad application prospects in the fields of prevention and treatment of cardiovascular and cerebrovascular diseases, tumors, and diabetes. However, anthocyanins can show significant efficacy only after reaching a ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62
CPCC07D311/62Y02P20/54
Inventor 李良玉孙蕊刁静静贾鹏禹李朝阳牛广财曹龙奎
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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