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A Beijing Paecilomyces strain lyz7 and its application

A technology of Paecilomyces pseudomycetes and strains, applied in the field of microorganisms, can solve the problems of limited degradation effect and insufficient degradation of tetracycline, and achieve the effects of reduction promotion, significant degradation effect, and remarkable degradation effect.

Active Publication Date: 2021-06-15
BEIJING DONGXIANG ENVIRONMENTAL TECH CO LTD
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AI Technical Summary

Problems solved by technology

[0005] However, it is far from enough to rely on these strains to degrade tetracycline. On the one hand, the degradation effect is limited, and on the other hand, the selection is also limited. Therefore, it is urgent to develop more new strains in this field, and even find new strains for effective degradation. tetracycline

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  • A Beijing Paecilomyces strain lyz7 and its application
  • A Beijing Paecilomyces strain lyz7 and its application
  • A Beijing Paecilomyces strain lyz7 and its application

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experiment example

[0065] Experimental example, screening and verification process of strains of the present invention

[0066] 1 Materials and methods

[0067] 1.1 Materials

[0068] 1.1.1 Source of strains

[0069] The soil samples used in the experiment were taken from the humus soil under the trees of Beijing Agricultural College.

[0070] 1.1.2 Culture medium

[0071] Basic inorganic salt medium: CaCl 2 , 0.02g; (NH 4 ) 2 SO 4 , 1.0g; NaCl, 0.5g; FeCl 3 , 0.05g; K 2 HPO 4 , 1.0g; NH 4 NO 3 , 1.0g; KH 2 PO 4 , 1.0 g; pH=7.0;

[0072] Trace element solution: CoCl 2 ·6H 2 O, 2g; MnCl 2 ·4H 2 O, 8.5 g; ZnCl 2 , 0.1g; NiCl 2 ·6H 2 O, 0.2 g; CuSO 4 ·5H 2 O, 0.3 g; Na 2 MoO 4 ·2H 2 O, 0.2 g; Na 2 SeO 4 ·2H 2 O, 0.2g to a 1L volumetric flask for later use;

[0073] Screening medium: add tetracycline to sterilized inorganic salt medium, and configure different concentrations according to the needs of the test; the addition amount of trace elements is 1 mL·L -1 ;

[00...

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Abstract

The invention "a Paecilillium pekinense strain LYZ7 and its application" belongs to the field of microorganisms. The preservation number of the strain LYZ7 is CGMCC No.16487. The present invention unexpectedly finds that the bacterial strain belongs to a new strain, and names the strain as Paecilomyces Simplicillium pekinense. Based on the bacterial strain, the present invention also provides a tetracycline-degradable bacterial agent, a tetracycline biodegradation agent and a tetracycline biodegradation method. The bacterial strain LYZ7 of the present invention has a very significant degradation effect on tetracycline, and after 14 days of treatment, the degradation rate is as high as 89.21%.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Paecilomyces beijingensis strain LYZ7 and its application. Background technique [0002] In recent years, with the development of my country's aquaculture industry, tetracycline antibiotics have been widely used due to their low price and remarkable effect. Due to the low absorption rate of these antibiotics in animals, most of them will enter the environment with animal excrement and accumulate in the environment, posing a threat to environmental safety and human health. [0003] There are many ways to deal with antibiotic pollution, mainly divided into abiotic degradation and biodegradation. Abiotic degradation mainly degrades antibiotics by physical or chemical means, such as photolysis, hydrolysis and oxidative degradation. Although abiotic degradation can quickly remove antibiotic residues, the chemical materials used for antibiotic degradation can hav...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C02F11/02C02F3/34B09C1/10C12R1/645C02F103/20
CPCB09C1/105C02F3/34C02F11/02C02F2103/20C12N1/145C12R2001/645
Inventor 刘悦秋尹静冯佳宁
Owner BEIJING DONGXIANG ENVIRONMENTAL TECH CO LTD
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