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Construction method and detection method of uplc characteristic map of Cnidium chinensis medicinal material

A technology of characteristic map and construction method, which is applied in the construction method and detection field of UPLC characteristic map of Cnidium chinensis medicinal material, can solve the problems that cannot fully reflect the quality and cannot fully reflect the material basic characteristics of traditional Chinese medicine decoction, so as to improve the quality control level, And stable inner quality, characteristic strong effect

Active Publication Date: 2021-11-16
GUANGDONG YIFANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The 2015 edition of "Chinese Pharmacopoeia" uses osthole as the quality evaluation index of osthole, and only controls the content of osthole, which cannot fully reflect its quality
At present, most of the fingerprints of Fructus Cnidii reported in the literature use the conventional HPLC method, and all of them only aim at the material basis of the original medicinal materials, and the index components mostly use fat-soluble components as the research objects; Qualitative identification of quality differences cannot fully reflect the material basis characteristics of traditional Chinese medicine decoctions

Method used

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  • Construction method and detection method of uplc characteristic map of Cnidium chinensis medicinal material
  • Construction method and detection method of uplc characteristic map of Cnidium chinensis medicinal material
  • Construction method and detection method of uplc characteristic map of Cnidium chinensis medicinal material

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Embodiment 1

[0063] This embodiment is a method for constructing the UPLC characteristic map of Cnidium chinensis medicinal material.

[0064] The main components of Cnidium Fructus are coumarin compounds, including osthole, bergamot lactone, xanthotoxin, imperatorin, etc. In order to maintain the consistency of the quality standards of Cnidiola Fructus and clinical decoction, and comprehensively control the quality of Cnidiola Fructus medicinal materials, the characteristic map of Cnidiola Fructus medicinal materials was established, and the established analysis method was verified.

[0065] 1. Instruments, reagents and reagents

[0066] Instrument: Waters ultra-high performance liquid chromatography (Waters, Waters H-class), TUV detector (Waters); Empower workstation; 1 / 10,000 balance (Mettler-Toledo, ME204E) One-millionth balance (Mettler-Toledo, XP26); ultrapure water machine (Merck, Milli-Q Direct 8 / 16system); ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd., KQ5500DE) ; ...

Embodiment 2

[0218] The present embodiment is a detection method of Fructus Cnidii medicinal material, and the steps are as follows:

[0219] 1. Chromatographic conditions

[0220] With YMC C18 (100mm × 2.1mm, 1.9 μm) as the chromatographic column, the injection volume: each 1 μL of the reference substance solution and the test solution; with methanol as the mobile phase A and aqueous solution as the mobile phase B, the following table (Table 5) provides gradient elution; the flow rate is 0.20 mL per minute; the column temperature is 30° C.; the detection wavelength is 310 nm. The number of theoretical plates should not be less than 6000 based on the peak of osthole.

[0221] table 5

[0222]

[0223] 2. Preparation of the sample solution: Take about 0.3 g of Cnidium chinensis medicinal material powder to be tested (passed through a No. 3 sieve) (batch number: G1712163), accurately weigh it, place it in a stoppered Erlenmeyer flask, add 20 mL of 80% methanol precisely, and weigh it. ...

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Abstract

The invention relates to a construction method and a detection method of a UPLC characteristic map of Cnidium chinensis medical material. The characteristic map construction method comprises the following steps: using imperatorin reference substance, osthole reference substance, xanthoxylin reference substance, and bergamot lactone as reference substances to prepare a reference substance solution; taking Cnidium chinensis medicinal material and standard decoction samples respectively , add extraction solvent to extract, filter, and the gained filtrate is used as need testing solution and standard decoction need testing solution; Draw described reference substance solution, need testing solution, standard decoction need testing solution respectively, inject ultra-high performance liquid phase Chromatography, determination; compare the obtained test sample spectrum with the standard decoction test sample spectrum, demarcate the water-soluble common peaks, and obtain the UPLC characteristic spectrum of Cnidinia Cnidii medicinal materials. The characteristic chromatogram can be used for qualitative and quantitative analysis of the quality of the Cnidium mongolicum medicinal material, can ensure the quality of the traditional Cnidinia decoction prepared by the medicinal material, and is also suitable for detecting other preparations containing the Cnidiac fruit.

Description

technical field [0001] The invention relates to the research on the quality of Chinese medicinal materials, in particular to a construction method and detection method of a UPLC characteristic map of the medicinal material Cnidium mongolica. Background technique [0002] Cnidium monnieri is the fruit of Umbelliferae Cnidium monnieri (L.) Cuss. "Shen Nong's Materia Medica" listed it as the top grade, and the fruit of Cnidium is used as medicine. Fructus Cnidii is an insecticidal and antipruritic drug, pungent, bitter, warm; slightly poisonous. Return the kidney channel. Efficacy can dry damp and dispel wind, kill insects and relieve itching, warm kidney and strengthen yang. For vaginal itching, eczema itching, arthralgia from dampness lumbago, kidney deficiency impotence, uterine cold infertility. Modern pharmacological studies believe that Cnidium is effective in antiarrhythmia, learning and memory, antagonizing hormone-induced osteoporosis, anti-mutagenesis, anti-tumor,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/36
CPCG01N30/02G01N30/36
Inventor 魏梅彭劲源霍文杰程学仁李乐黎晓丽梁慧何广铭朱德全陈向东
Owner GUANGDONG YIFANG PHARMA