A cosmetic composition comprising osmanthus asiaticus extract and clematis apiifolia extract
一种化妆品组合物、有效成分的技术，应用在化妆品、化妆品配制品、梳妆用配制品等方向，能够解决痛，面神经麻痹，中风、偏头痛，肌肉麻痹，类风湿关节炎、膝盖发酸并疼痛、腰疼、通风、手脚发麻、骨鲠在喉、改善活性未知晓等问题，达到抗氧化及美白效果优秀、抗氧化效果优秀的效果
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preparation example Construction
[0059] The preparation of the above-mentioned extract can be as follows, that is, after washing the extracted raw material with water, drying and pulverizing, and using a solvent whose volume is 8 times to 12 times the weight of the raw material, common methods such as reflux circulation, pressurized extraction, ultrasonic extraction Wait for extraction and filtration for 1 hour to 24 hours. Moreover, the above-mentioned extracts can also be obtained in powder form through additional processes, such as vacuum distillation or freeze-drying.
[0060] The above-mentioned extract may also include an extract constituted by usual purification. For example, the above-mentioned extract may also contain fractions obtained by additionally performing various purification methods, i.e., separation using an ultrafiltration membrane with a defined molecular weight cut-off, based on various chromatography (for use according to size, charge, Hydrophobic or affinity separation) separation, et...
preparation example
[0080] Preparation Example: Preparation of Natural Extract
[0081] After cleaning the silver osmanthus, clematis, Magnolia officinalis, Bletilla striata, Gallnut, Acanthopanax root, Cyperus cyperi, Morus alba bark, Perilla leaf, Purslane, Cortex Phellodendron and Polygonum multiflorum, completely dry at room temperature and pulverize, 100 g of pulverized products were obtained. 100 g of the pulverized product was extracted by immersing 80% ethanol at 20 times its volume at room temperature for 7 days.
[0082] The above-mentioned extract was filtered through a 250 mesh and 3 μm filter, concentrated under reduced pressure at a temperature of 40° C., and then freeze-dried to obtain a solid content of the extract.
[0083] Examples and Comparative Examples
[0084] In order to examine the skin improvement effect of the samples obtained, Examples and Comparative Examples were set by mixing samples as shown in Table 1 and Table 2 below.
[0085] Table 1
[0086] Content (p...
experiment example 1
[0092] Experimental Example 1: Skin Stability Test
[0093] In order to examine the skin stability of the samples of Examples and Comparative Examples, a cell viability assay (MTT assay) experiment was performed on fibroblasts (HDF), melanocytes (B16F10), and keratinocytes (HaCaT).
[0094] After suspending the samples of Examples and Comparative Examples in distilled water according to the concentration of 0mg / L, 1mg / L, 5mg / L, 10mg / L, 20mg / L, 50mg / L and 100mg / L respectively, the cell viability was measured.
[0095] The modification utilizes 3-(4,5-dimethylthiazol-2)-2,5-diphenyltetrazolium bromide (MTT{3-(4,5-dimethylthiazol-2-yl)-2-5- diphenyltetrazolium bromide}) reagent to measure cell viability Mosmann's (Mosmann's) method is modified to carry out cytotoxicity.
[0096] 1 x 10 in 96-well plate 4 The concentration of cells / well (cells / well) was divided into fibroblasts, melanin-forming cells and keratinocytes respectively, and the temperature was 37 ℃, 5% CO 2 condit...
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