Preparation method and application of a kind of mycoplasma avium culture medium and mycoplasma avium bacterial liquid

A technology of avian mycoplasma and liquid culture medium, which is applied in the field of avian mycoplasma culture medium, can solve the problems of unstable vaccine product quality, low antigen content and purity, low concentration of cultured bacterial liquid, etc., so as to reduce the probability of vaccine immune side reactions, The effect of saving labor and high antigen purity

Active Publication Date: 2022-01-14
RINGPU (BAODING) BIOLOGICAL PHARMACEUTICAL CO LTD
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the production of mycoplasma avium vaccine mainly uses improved Frey's medium, CM2 medium, etc. to cultivate, and these mediums have a higher serum concentration (generally more than 10%), and the concentration of the cultured bacteria solution is not high (generally 10%). 8-10 CCU / ml), the strains need to be reproduced for each production, the production operation is cumbersome, the cost is high, the quality of the vaccine product is unstable (antigen content and purity are not high), and side reactions are prone to occur

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method and application of a kind of mycoplasma avium culture medium and mycoplasma avium bacterial liquid
  • Preparation method and application of a kind of mycoplasma avium culture medium and mycoplasma avium bacterial liquid
  • Preparation method and application of a kind of mycoplasma avium culture medium and mycoplasma avium bacterial liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A kind of mycoplasma avium culture medium of the present invention, described mycoplasma avium culture medium is made up of mycoplasma avium basal culture medium and auxiliary component, and described basal culture medium is conventional liquid culture medium of mycoplasma avium, and described auxiliary component is composed of gelatin, Composed of Tween 80 and calcium chloride.

[0025] The basal medium is modified Frey's medium.

[0026] Described auxiliary component consumption is the following component composition of finished medium mass volume ratio (m:V):

[0027] Gelatin 0.2-0.4%,

[0028] Tween 80 0.5-1%,

[0029] Calcium chloride 0.2-0.4%.

[0030] A kind of preparation method of mycoplasma avium bacterium liquid of the present invention, the bacterial classification used for production is mycoplasma gallisepticum or mycoplasma gallisepticum, and the bacterium liquid comprises the following steps:

[0031] (1) Inoculate the qualified seeds for Mycoplasma a...

Embodiment 2

[0037] The difference between embodiment 2 and embodiment 1 is: a kind of mycoplasma avium culture medium of the present invention, described mycoplasma avium culture medium is made up of mycoplasma avium basal culture medium and auxiliary component, and described basal culture medium is conventional mycoplasma avium liquid Medium, the auxiliary components are composed of gelatin, Tween 80 and calcium chloride.

[0038] The basal medium is CM medium.

[0039] Described auxiliary component consumption is the following component composition of finished medium mass volume ratio (m:V):

[0040] Gelatin 0.2-0.4%,

[0041] Tween 80 0.5-1%,

[0042] Calcium chloride 0.2-0.4%.

[0043] A kind of preparation method of mycoplasma avium bacterium liquid of the present invention, the bacterial classification used for production is mycoplasma gallisepticum or mycoplasma gallisepticum, and the bacterium liquid comprises the following steps:

[0044] (1) Inoculate the qualified seeds for...

Embodiment 3

[0048] The difference between embodiment 3 and embodiment 1 is: a kind of mycoplasma avium culture medium of the present invention, described mycoplasma avium culture medium is made up of basic culture medium of mycoplasma avium and auxiliary component, and described basic culture medium is conventional mycoplasma avium liquid Medium, the auxiliary components are composed of gelatin, Tween 80 and calcium chloride.

[0049] The basal medium is conventional CM2 liquid medium.

[0050] Described auxiliary component consumption is the following component composition of finished medium mass volume ratio (m:V):

[0051] Gelatin 0.2-0.4%,

[0052] Tween 80 0.5-1%,

[0053] Calcium chloride 0.2-0.4%.

[0054] A kind of preparation method of mycoplasma avium bacterium liquid of the present invention, the bacterial classification used for production is mycoplasma gallisepticum or mycoplasma gallisepticum, and the bacterium liquid comprises the following steps:

[0055] (1) Inoculate...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a culture medium for avian mycoplasma, a preparation method for avian mycoplasma bacterial liquid and an application thereof, belonging to the field of biotechnology. The mycoplasma avium culture medium of the present invention is made up of basal culture medium and auxiliary component, and described basal culture medium is conventional liquid culture medium of mycoplasma avium, and auxiliary component is gelatin, Tween 80 and calcium chloride; Mycoplasma septicum or Mycoplasma gallinarum is inoculated, cultivated and harvested with bacterial liquid to produce Mycoplasma avium bacterial liquid to obtain Mycoplasma avium vaccine. The preparation method of the invention increases the antigen content of the mycoplasma avium bacterial liquid, simplifies the production process, reduces the production cost, reduces the occurrence probability of vaccine immunization side effects, and improves the vaccine immunization effect.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a preparation method of a mycoplasma avium culture medium, a mycoplasma avium bacterial liquid and an application thereof. Background technique [0002] Avian Mycoplasmosis (Avian Mycoplasmosis, AM) is an infectious disease of poultry caused by Mycoplasma avium. The common clinical pathogens are Mycoplasma Gallisepticum (MG) and Mycoplasma Synoviae (MS). Mycoplasma gallisepticum infection can cause Chronic Respiratory Disease (CRD), air sacculitis and sinusitis in poultry; Mycoplasma gallisepticum infection can cause symptoms such as joint swelling, synovial bursa and tendon inflammation, and parenchymal organ enlargement; Effective vaccine immunization is the main means of preventing and controlling the disease. [0003] At present, the production of mycoplasma avium vaccine mainly uses improved Frey's medium, CM2 medium, etc. to cultivate, and these mediums have a higher s...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K39/02A61P31/04C12R1/35
CPCA61K39/0241A61P31/04C12N1/20A61K2039/552A61K2039/522
Inventor 何平有朱秀同赵玉龙刘涛郁宏伟高晓磊焦玉兰刘浩邱贞娜张新新李卫东赵丽霞
Owner RINGPU (BAODING) BIOLOGICAL PHARMACEUTICAL CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap