Method for obtaining transcriptome and functional genes of blumea balsamifera

A functional gene and transcriptome technology, applied in genetic engineering, plant genetic improvement, chemical instruments and methods, etc., can solve the problems of slow progress in genetic research and lack of genetic information in Ainaxiang

Pending Publication Date: 2019-03-05
TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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Problems solved by technology

However, at present, there are few studies on the molecular biology of Ainaxiang, and the genetic information is very scarce, which makes the research progress of Ainaxiang gene level very slow.

Method used

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  • Method for obtaining transcriptome and functional genes of blumea balsamifera
  • Method for obtaining transcriptome and functional genes of blumea balsamifera
  • Method for obtaining transcriptome and functional genes of blumea balsamifera

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Embodiment Construction

[0017] The present invention will be further described below in conjunction with specific embodiments with reference to the accompanying drawings, so as to better understand the present invention.

[0018] RNA-seq analysis sample processing and sequencing process see figure 1 . The specific method is as follows:

[0019] 1. Extraction of the total RNA of Ainaxiang, eukaryotes build a library with an initial amount of 5ug total RNA;

[0020] 2. After the mRNA is separated by the magnetic bead method, the ion interrupts the mRNA (Truseq TM RNA sample prep Kit);

[0021] 3. Recover 200-700bp fragments, synthesize double-stranded cDNA, make up, add A to the 3' end, and connect the index adapter (Truseq TM RNAsample prep Kit);

[0022] 4. Library enrichment, PCR amplification for 15 cycles;

[0023] 5. 2% agarose gel recovery target band (Certified Low Range Ultra Agarose);

[0024] 6. Quantitative TBS380 (Picogreen), mixed according to the data ratio on the machine;

[00...

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Abstract

The invention provides a method for obtaining transcriptome and functional genes of blumea balsamifera. The method comprises (a) extracting the total RNA(ribonucleic acid) of blumea balsamifera, separating out mRNA (messenger RNA) with polyA (polyadenylation) at the 3' end, randomly breaking the mRNA, performing inverse transcription to synthesize double-stranded cDNA (complementary DNA); (b) sequencing an obtained sequence; (c) splicing and assembling a sequencing result to obtain Unigene and determining the orientation of the Unigene; (d) performing bioinformatics analysis on an obtained gene transcript to obtain the transcriptome and the function genes of blumea balsamifera. The method for obtaining the transcriptome and the functional genes of blumea balsamifera can help obtain 48197273 pieces of sequence information and 100341 Unigenes, involving 60477 pieces of information including RNA-seq names, sequence length and expression quantity, COG (cluster of ortholog genes) predication, COG functional annotations, KEGG (Kyoto encyclopedia of genes and genomes) annotations, KEGG-pathway and GO (gene ontology) annotations, and 37283 pieces of information protein function annotationsfor performing CDS (coding sequence) prediction on the obtained sequence information.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to the transcriptome information, functional gene expression level and annotation information of Ainaxiang, and their obtaining methods. Background technique [0002] Blumea balsamifera (Blumea balsamifera L.DC.) is a perennial woody herbaceous plant belonging to the family Asteraceae, mainly distributed in Hainan, Guizhou, Guangxi, Guangdong, Yunnan, Taiwan and other provinces in China. The whole herb or the ground part is used as medicine, which has the effects of analgesia, sweating, wind and dampness, phlegm and cough, menstrual flow and hemostasis. It has a long history of medicine in minority areas such as Li, Miao, and Zhuang. It is an important folk medicine. drug. At the same time, Ainaxiang is also one of the important plant sources for obtaining natural borneol (Ai Pian). Moreover, the moxa oil produced in the process of refining moxa tablets has the effects of d...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10C12N15/29
CPCC12Q1/6895C07K14/415C12Q1/6869
Inventor 官玲亮庞玉新张影波王凯陈振夏黄梅谢小丽胡璇
Owner TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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