Method for establishing an HPLC (high performance liquid chromatography) fingerprint of rhizoma dioscoreae nipponicae, standard fingerprint of Dioscorea nipponica Makino and application of standard fingerprint

A high-performance liquid chromatography and standard fingerprint technology, which is applied in measuring devices, instruments, scientific instruments, etc., to achieve the effects of reliable stability, easy mastery and strong repeatability

Inactive Publication Date: 2019-03-15
XUZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research report on the fingerprint of the pangolin in the literature. Therefore, in order to scientifically and effectively control its internal quality, it is necessary to establish the fingerprint of the pangolin

Method used

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  • Method for establishing an HPLC (high performance liquid chromatography) fingerprint of rhizoma dioscoreae nipponicae, standard fingerprint of Dioscorea nipponica Makino and application of standard fingerprint
  • Method for establishing an HPLC (high performance liquid chromatography) fingerprint of rhizoma dioscoreae nipponicae, standard fingerprint of Dioscorea nipponica Makino and application of standard fingerprint
  • Method for establishing an HPLC (high performance liquid chromatography) fingerprint of rhizoma dioscoreae nipponicae, standard fingerprint of Dioscorea nipponica Makino and application of standard fingerprint

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Fingerprints of the same batch of Pangosia chinensis

[0024] 1. Test equipment: WATERS 2695 high performance liquid chromatograph; WATERS 2998PAD ultraviolet detector, including online vacuum degasser, high-pressure binary gradient pump, constant temperature autosampler, and column oven.

[0025] 2. Reagents: acetonitrile (chromatographically pure), ultrapure water.

[0026] 3. High performance liquid chromatography conditions: Chromatographic column: XBridge C18 (250mm×4.6mm, 5μm); flow rate is 1.0ml / min; injection volume is 10μl; detection wavelength is 208nm; column temperature is 30°C; mobile phase is acetonitrile (A)-Water (B), the method of gradient elution is 0~5min, 5%A; 5~15min, 5%~15%A; 15~55min, 15%~45%A; 55~65min, 45%~100%A; 65~80min, 100%A.

[0027] 4. Preparation of the test product solution: take the medicinal material of pangolinia, crush it and pass it through a 50-100 mesh sieve, accurately weigh 1g of the sieved pangolin powder, put it i...

Embodiment 2

[0045] Example 2: Fingerprints of 11 batches of Pangosia chinensis from different origins

[0046] (1) Determination of standard fingerprints: Take 11 batches of Pangosia chinensis medicinal materials from different origins such as Jilin, Anhui, Hebei, Liaoning, etc., and perform high-performance liquid chromatography analysis according to the detection conditions of Example 1, and obtain the HPLC fingerprints of 11 batches of samples, Such as figure 2 shown. Through the analysis and comparison of the HPLC fingerprints of 11 batches of pangolins from different origins, the similarity evaluation was carried out, and a total of 21 common characteristic peaks were determined. The chromatographic peak No. For the control peak, calculate the relative retention time of other 20 common characteristic peaks, which are as follows: the relative retention time of peak No. 1 is 0.591, the relative retention time of peak No. 2 is 0.649, the relative retention time of peak No. The retent...

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Abstract

The invention discloses a method for establishing an HPLC (high performance liquid chromatography) fingerprint of rhizoma dioscoreae nipponicae, a standard fingerprint of rhizoma dioscoreae nipponicaeand an application of the standard fingerprint. The method comprises the following steps: (1) taking and soaking Dioscorea nipponica Makino medicinal powder in methanol, performing ultrasonic extraction, filtering a supernate with a microporous filter membrane, and taking a filtrate as a test solution; (2) performing HPLC analysis under conditions that the chromatographic column is XBridge C18 (250 mm*4.6 mm, 5 mu m), flow rate is 1.0 ml / min, sample size is 10 mu l, detection wavelength is 208 nm, column temperature is 30 DEG C, the mobile phase is acetonitrile (A)-water (B), and gradient elution procedure is 5% of A for 0-5 min, 5%-15% of A for 5-15 min, 15%-45% of A for 15-55 min, 45%-100% of A for 55-65 min and 100% of A for 65-80 min; drawing the test solution for injection to obtainthe HPLC fingerprint of rhizoma dioscoreae nipponicae. The rhizoma dioscoreae nipponicae standard fingerprint established with the method has 21 common characteristic peaks, and quality of the rhizomadioscoreae nipponicae medicinal material can be effectively monitored by the standard fingerprint.

Description

technical field [0001] The invention belongs to the technical field of detection of traditional Chinese medicines, and in particular relates to a method for establishing a high-efficiency liquid chromatography fingerprint of Chuanshanlong and its standard fingerprint and application. Background technique [0002] Dioscorea nipoonica, also known as Dioscorea nipoonica Makino, is the dry rhizome of Dioscorea nipoonica Makino, a perennial herbaceous vine. The rhizome of Pangosaurus mainly contains steroidal saponins, and its aerial part mainly contains phenanthrene and flavonoids. It has the effects of relaxing tendons and activating collaterals, dispelling wind and relieving gout, treating damp arthralgia and joint swelling. Modern pharmacological studies have shown that it has the effects of lowering blood sugar, regulating immunity, improving cardiovascular function, and anti-tumor. [0003] The 2015 edition of the Chinese Pharmacopoeia has determined the content of diosge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 卜伟
Owner XUZHOU NORMAL UNIVERSITY
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