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Application of cd317 inhibitor in preparation of medicine for treating liver cancer

A technology for treating liver cancer and inhibiting liver cancer, which is applied in drug combinations, pharmaceutical formulations, anti-tumor drugs, etc., to achieve the effect of inhibiting the cell cycle transition of liver cancer cells and inhibiting the proliferation of cancer cells

Active Publication Date: 2021-05-14
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

(Swiecki, M., etal.(2013). "BST-2 / tetherin: structural biology, viral antagonistism, and immunobiology of a potent host antiviral factor." Mol Immunol 54(2):132-139.), CD317 overexpression Promote malignant behaviors such as tumor cell proliferation, metastasis and apoptosis resistance 2009,9:102.2.Sayeed, A., et al.AberrantRegulation of BST2(Tetherin) Promoter Enhances Cell Proliferation and Apoptosis Evasion in High Grade Breast Cancer Cells.Plos One, 2013.8(6).), but before the publication of the present invention, Whether it is possible to directly regulate the expression of CD317 to treat liver cancer still lacks clear conclusions and direct experimental evidence

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  • Application of cd317 inhibitor in preparation of medicine for treating liver cancer
  • Application of cd317 inhibitor in preparation of medicine for treating liver cancer
  • Application of cd317 inhibitor in preparation of medicine for treating liver cancer

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Embodiment

[0026] 1. siRNA design and transfection

[0027] According to the basic principle of siRNA target sequence, a siRNA sequence of 21 nucleotides was designed for the human CD317 gene transcript (NM_004335.3), that is, siCD317, including the sense strand and the antisense strand, and its base sequence is as follows:

[0028] Sense strand: 5'-CCAGGUCUUAAGCGUGAGAdTdT-3', antisense strand: 5'-UCUCACGCUUAAGACCUGGdTdT-3';

[0029] The base sequence of the negative control (NC) siRNA selected in this embodiment is as follows:

[0030] Sense strand: 5'-UUCUCCGAACGUGUCACGUdTdT-3', antisense strand: 5'-ACGUGACACGUUCGGAGAAdTdT-3';

[0031] Two deoxyribonucleotides in a single-chain suspension structure are added to the 3' end of the interference fragment in the present invention to enhance the stability of siRNA in vivo and in vitro and prevent degradation. The siRNA was synthesized by Shanghai Gemma Company.

[0032] According to a preferred embodiment of the present invention, Lipofec...

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Abstract

The invention relates to the field of genetic engineering medicines, in particular to the application of CD317 inhibitors in the preparation of medicines for treating liver cancer. The present invention provides the inhibitory effect of CD317 inhibitor on the proliferation of liver cancer cells and the inhibitory effect on the transition of liver cancer cell cycle, thereby proving the therapeutic effect of CD317 inhibitor on liver cancer. The results showed that CD317 siRNA could effectively silence the expression of CD317 in liver cancer cells, significantly inhibit the cell cycle transition of liver cancer cells and inhibit the proliferation of cancer cells.

Description

technical field [0001] The invention relates to the field of genetic engineering drugs, in particular to the application of CD317 inhibitors in the preparation of drugs for treating liver cancer. Background technique [0002] Liver cancer refers to malignant tumors that occur in the liver, including primary liver cancer and metastatic liver cancer. People usually refer to primary liver cancer when they say liver cancer. Primary liver cancer (primary carcinoma of liver) refers to cancer that occurs in liver cells or intrahepatic cholangiocytes. Among them, hepatocellular carcinoma is a malignant tumor that seriously affects human life and health, and its morbidity and mortality rank fifth and third among all tumors in the world (A. Jemal, F. Bray, M.M. Center, J. Ferlay, E. Ward, D. Forman, Global cancer statistics, CA: a cancer journal for clinicians, 61 (2011) [0003] 69-90.). Hepatocellular carcinoma progresses rapidly, has a short course of disease, and is difficult t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K45/00A61K31/7088A61P1/16A61P35/00
CPCA61K31/7088A61K45/00A61P1/16A61P35/00
Inventor 万晓春陈有海章桂忠刘曌刘绿艳
Owner SHENZHEN INST OF ADVANCED TECH