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Molecular marker associated with properties of semen volume of boars and application

A molecular marker, semen volume technology, applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc. Simple, specific results

Active Publication Date: 2019-04-02
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The abnormal factors of boars are eliminated, which reduces the production efficiency of boars and increases the cost of breeding boars

Method used

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  • Molecular marker associated with properties of semen volume of boars and application
  • Molecular marker associated with properties of semen volume of boars and application
  • Molecular marker associated with properties of semen volume of boars and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Genotyping Detection

[0030] (1) Use the conventional magnetic bead method sperm genome extraction kit (Wuhan Nanomagnetic Biotechnology Co., Ltd.) to automatically extract the total DNA of Duroc boar sperm. The specific steps are:

[0031] (1) Take an appropriate amount of semen (5-15 μL) from a Duroc boar into a 1.5 mL centrifuge tube;

[0032] (2) Add 500 μL of lysate (included in the above kit) and 5 μL of proteinase K (20 mg / mL) to the centrifuge tube, shake and mix for 30 seconds, place in a 65°C oven or metal bath, and lyse for 30 minutes to 1 hour;

[0033] (3) After the lysis is completed, transfer all the supernatant to a deep-well plate (marked as ①), and add 350 μL of isopropanol to each well;

[0034] (4) Put the No. ① deep-well plate on station 1 of the nucleic acid extractor, and place the deep-well plates with magnetic beads, washing solution ①, washing solutions ② and ③ and eluent on station 2~ 6 on. Turn on the power of the instrument, a...

Embodiment 2

[0042] Example 2: Application of the WU_10.2_13_11960687 molecular marker typing method in the correlation analysis of semen volume traits in Duroc boars

[0043] (1) Phenotypic pretreatment of semen traits of Duroc boars

[0044] The traits of boar semen belong to repeated measurement traits. It is necessary to use the mixed linear model (MLM). The joint effect of boar station, year and month is used as the fixed effect, the age in days and semen collection interval are used as covariates, and the individual is used as a random effect to calculate the individual random effect. Effects, as newly constructed phenotypes (pseudo-phenotypes) for subsequent GWAS analysis. The lme4 package in the R statistical environment was used for analysis. The data included 2020 individuals and 105201 semen collection records. The specific model is as follows:

[0045]

[0046] Among them, y ijklm is the original phenotypic value of semen traits (sperm volume) of the lth individual at the...

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Abstract

The invention belongs to the technical field of screening of molecular markers, and particularly relates to a molecular marker associated with the properties of the semen volume of boars and an application. The molecular marker is cloned from genetic fragments of which the accession number is WU_10.2_13_11960687, the genes are typed and screened through a gene chip technique, and the molecular marker associated with the properties of the semen volume of boars is obtained. The nucleotide sequence of the marker is as shown in SEQID NO:1, a C / T allele mutant exists at the 101st basic group of thesequence, the mutant causes polymorphism of the nucleotide sequence, and when the 101st nucleotide in the SEQID NO:1 is T, the situation that the boar have more semen volume can be judged. The invention provides a new SNP molecular marker resource for auxiliary selection of markers for the properties of the semen volume of the boars.

Description

technical field [0001] The invention belongs to the technical field of porcine molecular marker screening, and specifically relates to a molecular marker associated with boar semen volume traits and an application thereof. The molecular marker relates to a SNP related to boar semen volume. The molecular marker of the invention can be used for marker-assisted selection of boar semen traits. Background technique [0002] According to the "2016-2017 China Pig Industry Yearbook", although 53.84% of domestic pig farms are smaller than 500 heads, 30.76% of pig farms are larger than 1,000 heads. In recent years, although small free-range farmers are still the main force in my country's pig industry, large-scale pig farming is the general trend of the development of China's pig industry. [0003] The application and promotion of pig artificial insemination technology can reduce the number of boar breeding in large-scale pig farms, which is of great significance for reducing the cos...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 项韬陈毅龙赵书红李新云刘阿丽刘向东施亮刘小磊梅全顺
Owner HUAZHONG AGRI UNIV
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