A micro-nanofluidic array chip reactor for high-throughput accurate comparative analysis and design of multi-cell, multi-microenvironment and multi-microecological factors

Abstract

A micro-nanofluidic array chip reactor for high-throughput accurate comparative analysis and design of multi-cell, multi-microenvironment, and multi-microecological factors adopts an embedded structure, and two layers of PDMS boards are bonded through cross-pipes. The PDMS A board forms a "two-input, four-output" and "three-input, four-output" structure through the input port and the micro-nanofluidic pipeline, and mixes 3 different factors and buffer solutions into 64 kinds containing factor 1, factor 2 and factor 3 mixed solutions. Each solution output port containing three factors is connected with three cell suspension input ports. Each type of cell is input from the input port of the PDMS B board, and flows into the corresponding cell culture chamber through the connecting port of the two-layer board. The chamber realizes the parallel culture of three cells of the same type. The invention is used to monitor the experimental research and detection analysis of the state and fate of multi-cells under the influence of multi-microenvironment, multi-microecological factors and dynamic concentration stimulation of control factors.

Description

technical field [0001] The invention belongs to the field of medical cell biology experiments and medical detection devices, and relates to a micro-nano fluidic array chip reactor for high-throughput accurate comparative analysis and design of multi-cells, multi-microenvironments, and multi-microecological factors. Background technique [0002] The interaction between body cells, their microenvironment and body microorganisms has become one of the research hotspots in the field of medical cell biology in recent years. Observing and detecting a series of changes in the cells of the body under the stimulation of dynamic concentrations of multiple factors is one of the common means to study the fate and state of the cells in the body. With its unique advantages, the micro-nanofluidic chip has become an ideal experimental platform for simulating the dynamic microenvironment of body cells, and has a wide range of applications in the field of medical cell biology research. Reason...

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Problems solved by technology

[0003] However, it is currently impossible to determine the optimal combination of external soluble factors and their optimal concentration ratio, which has become an important factor hindering the monitoring of the state and fate of the body's cells. Therefore, how to optimize the combination and concentration of soluble factors is the key to regulating the cell fate of the body. Key issues to be resolved with status

In the process of optimizing the combination of external solubility factors and their concentration ratios, most of the existing research uses a specific solubility factor or a specific concentration as the experimental condition alone, which is time-consuming and insufficient for the research. effective feedback

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