Detecting method of red-series coloring agents in medicinal materials
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A detection method and dye technology, applied in the field of analysis, can solve problems such as low detection efficiency, achieve the effects of rapid qualitative detection, reduce equipment requirements, and improve detection efficiency
Inactive Publication Date: 2019-04-05
KANGMEI PHARMA
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[0006] Most of the above detection methods are single dye detection, and the detection efficiency is low. Therefore, it is necessary to explore the method of detecting multiple components of the red series of Chinese medicinal materials at the same time, and establish a method that can detect multiple components of the red series of Chinese medicinal materials. Universal detection method for simultaneous detection of components
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Embodiment 1
[0060] The making of a kind of standard atlas of red staining agent comprises the following steps:
[0061] 1) Take carmine, amaranth, and erythrosin standard reagents 1 mg and add 10 mL of 70% ethanol to make the first standard solution, and each take acid red 73, new fuchsin, 808 scarlet, Sudan red Ⅰ, and Sudan red Ⅳ standard reagents 1mg plus 10mL 70% ethanol to make the second standard solution;
[0062] 2) Take 1 mL each of the first standard solution and the second standard solution in step 1), and dilute to 5 mL with 70% ethanol to obtain a composite standard solution;
[0063] 3) Use a 0.22 μm polytetrafluoroethylene filter membrane to filter, and inject the composite standard solution obtained in step 2) into a liquid chromatograph, and measure at a detection wavelength of 510 nm;
[0064] The parameters of the liquid chromatograph are:
[0065] Column: C 18 reversed-phase chromatographic column;
[0066] Mobile phase: A acetonitrile;
[0067] B 0.02mol / L ammonium ...
Embodiment 2
[0076] A detection method for a red coloring agent in madder medicinal material, comprising the following steps:
[0077] 1) Take 1 g of the sample to be tested, add 15 mL of 50vt% ethanol, ultrasonically extract for 10 minutes, centrifuge to take the supernatant, filter through a 0.45 μm filter membrane to obtain the solution to be tested;
[0078] 2) Absorb 10 μL of the standard solution in step 1) through a 0.22 μm polytetrafluoroethylene filter membrane, inject it into a liquid chromatograph, and perform elution at a detection wavelength of 510 nm;
[0079] Column: C 18 reversed-phase chromatographic column;
[0080] Mobile phase: A acetonitrile;
[0081] B 0.02mol / L ammonium acetate solution;
[0082] Elution program: t 0-15min , mobile phase A gradually increased from 5vt% to 15vt%;
[0083] t 15-40min , the proportion of mobile phase A increased by 60vt%;
[0084] t 40-41min , the proportion of mobile phase A increased by 10vt%;
[0085] t 41-50min , the propo...
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Abstract
The invention discloses a detecting method of red-series coloring agents in medicinal materials. The detecting method comprises the following steps: (1) standard reagents of carmine, amaranth and erythrosine are taken to be added with ethyl alcohol of 60-80%, a first standard solution is prepared, standard reagents of acid red 73, new fuchsine, 808 scarlet, Sudan I and Sudan IV are taken to be added with ethyl alcohol, and a second standard solution is prepared; (2) 1 mL of the first standard solution and 1 mL of the second standard solution in the step (1) are taken to be diluted, and a composite standard solution is obtained; (3) the composite standard solution obtained in the step (2) is taken to be injected into a liquid chromatograph to be measured through the detection wavelength of500-520 nm; and (4) a to-be-detected sample is taken to be analyzed, and suspected peaks are re-checked through a ultraviolet-visible light chromatograph. According to the detecting method, whether aproduct contains the red-series coloring agents or not can be quickly and qualitatively detected, all signal peaks are sharp and free of trailing and interference, requirements for equipment are lowered, the checkout efficiency is improved, and the detecting method has optimistic market prospects.
Description
technical field [0001] The invention relates to an analysis method, in particular to a detection method of a red coloring agent in medicinal materials. Background technique [0002] Adding dyes to traditional Chinese medicine is an illegal addition. Chinese herbal medicines are mainly tested for red series dyes: carmine, amaranth, erythrosin, acid red 73, new fuchsin, 808 scarlet, Sudan red Ι, and Sudan red Ⅳ. For this reason, the State Food and Drug Administration has issued a number of detection methods for the dyeing of Chinese medicinal materials, but these methods are mostly for the detection of single Chinese medicinal materials (see Table 1 below). When faced with a variety of Chinese medicinal materials, due to the diversity of methods , leading to the complexity of the pre-treatment and analysis process on the computer, and the slow detection efficiency. Therefore, a detection method of red dye in Chinese medicinal materials was explored to find a general detection...
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