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Immune cell amplification method and immune cell culture medium

An immune cell and cell culture technology, applied in the field of cell culture, can solve the problems of weak tumor cell killing ability, weak cell anti-apoptosis ability, short survival time, etc., to shorten the culture period, strong anti-apoptosis ability, long-lived effect

Pending Publication Date: 2019-04-09
GUANGDONG JIDE KANGMIN BIOTECHNOLOGY CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But the shortcoming of this method is: the cycle of cell culture expansion is about 14 days, and the survival time of the expanded people Vγ9Vδ2T cells is shorter (can continue to survive for about 7 days), and the anti-apoptotic ability of cells is not strong, which is harmful to tumor cells. The lethality is not strong, etc.

Method used

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  • Immune cell amplification method and immune cell culture medium
  • Immune cell amplification method and immune cell culture medium
  • Immune cell amplification method and immune cell culture medium

Examples

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Embodiment 1

[0092] This embodiment 1 provides an immune cell culture medium, including RPMI-1640 medium and interleukin-2, interleukin-15 and vitamin E added to the RPMI-1640 medium.

[0093] Wherein, in the immune cell culture medium, the concentration of interleukin-2 is 200ng / ml, the concentration of interleukin-15 is 400ng / ml, and the concentration of vitamin E is 200mM.

Embodiment 2

[0095] This embodiment 2 provides an immune cell culture medium, including RPMI-1640 medium and interleukin-2, interleukin-15 and vitamin E added to the RPMI-1640 medium.

[0096] Wherein, in the immune cell culture medium, the concentration of interleukin-2 is 400ng / ml, the concentration of interleukin-15 is 500ng / ml, and the concentration of vitamin E is 300mM.

Embodiment 3

[0098] This embodiment 3 provides an immune cell stimulation expansion medium, including the immune cell culture medium as described in Example 1 and zoledronic acid added to the immune cell culture medium.

[0099] Wherein, in the immune cell stimulation expansion medium, the concentration of zoledronic acid is 200 μM.

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Abstract

The invention discloses an immune cell amplification method and an immune cell culture medium. Interleukin-15 and vitamin E are added in the amplification culture process of the human gamma9vdelta2T cell. Compared with traditional amplification methods, the immune cell amplification method has the advantages that proliferation efficiency and cell purity of the human gamma9vdelta2T cell can be improved, the cultured human gamma9vdelta2T cell has stronger anti-apoptosis capacity and longer cell survival time, the expression level of killer functional factors NKG2D, IFN-gamma, TNF-alpha and CD107a is higher, and thus, the killing capacity for tumor cells is higher.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to an immune cell expansion method and an immune cell culture medium. Background technique [0002] Immune cell therapy has become a new and important medical method for the treatment of refractory diseases (such as malignant tumors) in the medical field all over the world, such as the use of CAR-T cells for the treatment of B-cell lymphoma. For the scientific and medical communities, obtaining immune cells with stable and reliable quality and excellent functions is very critical to obtain good clinical therapeutic effects. [0003] Human Vγ9Vδ2 T cells are a kind of T cell subsets with anti-tumor and anti-infection functions that only exist in primates and humans. Human Vγ9Vδ2T cells can be used for immune cell therapy or immune function reconstruction of people suffering from tumors, refractory infectious diseases and immune dysfunction diseases. [0004] At present, there ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0783
CPCC12N5/0636C12N2500/38C12N2501/2302C12N2501/2315C12N2501/999
Inventor 吴扬哲何均仪
Owner GUANGDONG JIDE KANGMIN BIOTECHNOLOGY CO LTD
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