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Human Vgamma9Vdelta2T cell proliferation method and culture medium

A cell culture and culture medium technology, applied in the field of human Vγ9Vδ2T cell expansion method and culture medium, can solve the problems of short survival time, weak tumor cell killing ability, weak anti-apoptosis ability of cells, etc., and achieve long survival time , strong killing and inhibiting ability, strong anti-apoptotic ability

Active Publication Date: 2019-02-15
GUANGDONG JIDE KANGMIN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But the shortcoming of this method is: the cycle of cell culture expansion is about 14 days, and the survival time of the expanded people Vγ9Vδ2T cells is shorter (can continue to survive for about 7 days), and the anti-apoptotic ability of cells is not strong, which is harmful to tumor cells. The lethality is not strong, etc.

Method used

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  • Human Vgamma9Vdelta2T cell proliferation method and culture medium
  • Human Vgamma9Vdelta2T cell proliferation method and culture medium
  • Human Vgamma9Vdelta2T cell proliferation method and culture medium

Examples

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Embodiment 1

[0103] This embodiment 1 provides a human Vγ9Vδ2 T cell culture medium, including RPMI-1640 medium and interleukin-2, interleukin-15 and vitamin C added to the RPMI-1640 medium.

[0104] Wherein, in the human Vγ9Vδ2T cell culture medium, the concentration of interleukin-2 is 300 ng / ml, the concentration of interleukin-15 is 500 ng / ml, and the concentration of vitamin C is 100 mM.

Embodiment 2

[0106] This embodiment 2 provides a human Vγ9Vδ2 T cell culture medium, including RPMI-1640 medium and interleukin-2, interleukin-15 and vitamin C added to the RPMI-1640 medium.

[0107] Wherein, in the human Vγ9Vδ2T cell culture medium, the concentration of interleukin-2 is 500 ng / ml, the concentration of interleukin-15 is 700 ng / ml, and the concentration of vitamin C is 300 mM.

Embodiment 3

[0109] This embodiment 3 provides a human Vγ9Vδ2T cell stimulation and expansion medium, including the human Vγ9Vδ2T cell culture medium as described in Example 1 and zoledronic acid added to the human Vγ9Vδ2T cell culture medium.

[0110] Wherein, the concentration of zoledronic acid in the human Vγ9Vδ2T cell stimulation expansion medium is 300 μM.

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Abstract

The invention discloses a human Vgamma9Vdelta2T cell proliferation method and a culture medium. Interleukin-15 and vitamin C are added in the proliferation culture process of human Vgamma9Vdelta2T cells, compared with a traditional proliferation method, the method can improve the proliferation efficiency and purity of the human Vgamma9Vdelta2T cells, and the cultured human Vgamma9Vdelta2T cells which have higher anti-apoptosis capacity and longer cell survival time at the same time have higher anti-apoptosis capacity, longer cell survival time and higher expression level of key killing molecules NKG2D and thus have high killing capacity for tumor cells.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to a human Vγ9Vδ2 T cell expansion method and culture medium. Background technique [0002] Immune cell therapy has become a new and important medical method for the treatment of refractory diseases (such as malignant tumors) in the medical field all over the world, such as the use of CAR-T cells for the treatment of B-cell lymphoma. For the scientific and medical communities, obtaining immune cells with stable and reliable quality and excellent functions is very critical to obtain good clinical therapeutic effects. [0003] Human Vγ9Vδ2 T cells are a kind of T cell subsets with anti-tumor and anti-infection functions that only exist in primates and humans. Human Vγ9Vδ2T cells can be used for immune cell therapy or immune function reconstruction of people suffering from tumors, refractory infectious diseases and immune dysfunction diseases. [0004] At present, there are two ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0783
CPCC12N5/0636C12N2500/38C12N2501/999C12N2501/2315C12N2501/2302A61P35/00A61K35/17
Inventor 尹芝南吴扬哲徐艳
Owner GUANGDONG JIDE KANGMIN BIOTECHNOLOGY CO LTD
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