Pituitrin solution and preparation method thereof

A technology of vasopressin and vasopressin powder, which is applied in the field of medicine and can solve the problems of low potency and low process yield

Active Publication Date: 2019-04-12
SPH NO 1 BIOCHEM & PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a method for preparing pituitrin solution in order to overcome defects such as low yield and low potency of the existing pituitary preparation process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] 1. Grind the posterior pituitary granules into powder with a pulverizer.

[0044] 2. Get 3kg of the posterior pituitary gland powder, add 20L of 90% acetone, leave it to stand to remove the virus after stirring; and carry out the inactivation verification of the lipid envelope virus, the results show that PRV (pseudorabies virus, Pseudorabies virus) and VSV (vesicular stomatitis Virus, Vesicular Stomatitis Virus) indicates that the decrease in virus titer is ≥4.0logs.

[0045]3. Centrifuge (3000rpm, centrifuge for 40 minutes) to collect the precipitate, and then add 12L of 90% acetone to stir and centrifuge (3000rpm, centrifuge for 40 minutes).

[0046] 4. Repeat the above steps twice.

[0047] 5. Take the precipitate and add 4L of 100% acetone, stir and centrifuge (3000rpm, centrifuge for 40 minutes), weigh 2.8kg after the precipitate is dried.

[0048] 6. Add 15L 10°C 0.3% acetic acid (volume percentage) to the precipitate, stir and heat to 50°C, keep warm for 30 mi...

Embodiment 2

[0052] 1. Grind the posterior pituitary granules into powder with a pulverizer.

[0053] 2. Take 5kg of the posterior pituitary leaf powder, add 25L of 90% acetone, stir and let stand to remove the virus; and verify the inactivation of lipid enveloped virus, the results show that the PRV and VSV indicator virus titer drop value is ≥ 4.0logs.

[0054] 3. Centrifuge (5000rpm, centrifuge for 20 minutes) to collect the precipitate, then add 15L of 90% acetone to stir and centrifuge (5000rpm, centrifuge for 20 minutes).

[0055] 4. Repeat the above steps twice.

[0056] 5. Take the precipitate and add 5L of 100% acetone, stir and centrifuge (5000rpm, centrifuge for 20 minutes), weigh 4.9kg after the precipitate is dried.

[0057] 6. Add 20L of 0.3% acetic acid (volume percentage) at 2°C to the precipitate, stir and heat to 50°C, keep warm for 30 minutes and continue to heat to 95°C, add 900g chlorobutanol of analytical grade, continue to heat to boiling, and keep warm for 2 minute...

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PUM

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Abstract

The invention discloses a pituitrin solution and a preparation method thereof. The preparation method of the pituitrin solution comprises the steps that in an extraction process of powdered pituitrinon acetic acid, chloretone is added, and the addition amount of the chloretone is 18-35% by mass of the powdered pituitrin. The pituitrin solution prepared by the preparation method disclosed by the invention has high titer (as high as 18.3u / ml ), and in the virus inactivation process, active components (the yield is as high as 1281 thousands u / kg) cannot lose, so that the pituitrin solution moreconforms to the quantity standard of biological products.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a pituitary hormone solution and a preparation method thereof. Background technique [0002] Pituitrin is a water-soluble component extracted from the posterior pituitary gland of pigs and cattle, and contains oxytocin and vasopressin (also called vasopressin). Oxytocin has a selective effect on uterine smooth muscle, and its strength depends on the dose and the physiological state of the uterus. For the non-pregnant uterus, a small dose can strengthen the rhythmic contraction of the uterus; a large dose can cause the tonic contraction of the uterus. For the pregnant uterus, it is not sensitive in the early pregnancy, and the sensitivity gradually increases in the late pregnancy. The characteristics of this product on the uterus are: it has a strong contraction effect on the uterine body, but a small contraction effect on the cervix. [0003] In addition, it can a...

Claims

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Application Information

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IPC IPC(8): C07K7/16C07K1/14
CPCC07K7/16
Inventor 汪雅雯孙源远丁金国黄臻辉
Owner SPH NO 1 BIOCHEM & PHARMA CO LTD
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