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A functionalized gold nanoparticle and preparation method thereof, preparation method and application of gold nanoparticle dimer

A gold nanoparticle, functionalized technology, applied in metal processing equipment, organic chemistry, transportation and packaging, etc., can solve the problem of gold nanoparticles failing to study biological imaging

Active Publication Date: 2020-11-06
LINYI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no report on the use of click reaction chemistry for dark field imaging, and the existing gold nanoparticles have not been able to realize biological imaging research through click reaction chemistry.

Method used

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  • A functionalized gold nanoparticle and preparation method thereof, preparation method and application of gold nanoparticle dimer
  • A functionalized gold nanoparticle and preparation method thereof, preparation method and application of gold nanoparticle dimer
  • A functionalized gold nanoparticle and preparation method thereof, preparation method and application of gold nanoparticle dimer

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preparation example Construction

[0026] The invention provides a method for preparing functionalized gold nanoparticles, comprising the following steps:

[0027] Mix polyethylene glycol-gold nanoparticles, 2-aminoethanethiol and ester compounds, and perform amide reaction to obtain functionalized gold nanoparticles;

[0028] The ester compound contains an alkynyl group or an azido group;

[0029] The functionalized gold nanoparticles include alkyne functionalized gold nanoparticles or azido functionalized gold nanoparticles.

[0030] In the present invention, the ester compound is preferably azide-PEG4-NHS ester or compound 1. In the present invention, the structural formula of the compound 1 is:

[0031] The CAS number is 1174157-65-3.

[0032] The structural formula of the azide-PEG4-NHS ester is:

[0033] The CAS number is 944251-24-5.

[0034] In the present invention, the mass ratio of polyethylene glycol-gold nanoparticles, 2-aminoethanethiol and ester compounds is preferably 1:10:10. The pres...

Embodiment 1

[0056] Mix the PBS dispersion of PEG-AuNPs with 10 μL of 10 -7 M azide-PEG4-NHS ester aqueous solution was mixed, and the resulting system was subjected to amidation reaction at 25°C for 2 hours, the reaction product was washed with PBS solution, centrifuged, and the supernatant was removed, and the obtained material was dispersed in PBS solution to obtain Au- N 3 Dispersions;

[0057] The PBS dispersion of PEG-AuNPs was mixed with 2-aminoethanethiol aqueous solution (20 μL, 1×10 -7 M) Mix and carry out the bonding reaction at 25°C for 2h, then centrifuge the obtained product for 15min, wash the obtained centrifuged product, and add an aqueous solution of Compound 1 (3 μL, 1×10 -6 M), carry out amide reaction for 2h, wash the reaction product with PBS solution, centrifuge, remove the supernatant, and disperse the obtained material in the PBS solution to obtain the Au-C≡C dispersion;

[0058] Au-N 3 The dispersion (20 μL, 1 μM) was mixed with the Au-C≡C dispersion (20 μL, 1...

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Abstract

The invention belongs to the technical field of biological imaging, and in particular relates to a functionalized gold nanoparticle and a preparation method thereof, a preparation method of a gold nanoparticle dimer and an application thereof. The present invention provides a preparation method of functionalized gold nanoparticles. The present invention uses the prepared functionalized gold nanoparticles as a probe to form a gold nanoparticle dimer, and uses it to measure copper ions in rat serum. The determination of copper ions provides a new method, provides guidance for acute poisoning treatment of metal cations, and the invention realizes the use of click reaction chemistry for dark field imaging.

Description

technical field [0001] The invention relates to the technical field of biological imaging, in particular to a functionalized gold nanoparticle and a preparation method thereof, a preparation method of a gold nanoparticle dimer and an application thereof. Background technique [0002] The application of local surface plasmon resonance (LSPR) light scattering correlation method in chemistry, biomedicine, etc. is very promising, because LSPR endows light scattering with a characteristic band that is easy to distinguish from other particle scattering. LSPR-based dark-field microscopy (DFM) is a promising technique for non-destructive and non-photobleaching bioimaging. In recent years, researchers have done a lot of work to detect biomolecules or track dynamic biophysical processes by recording the wavelength changes of LSPR of noble metal nanoparticles by DFM. LSPR on the surface of noble metal nanoparticles has been extensively studied in fields such as biomedicine and optoele...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B22F1/00C07D207/46
CPCC07D207/46B22F1/054B22F1/145
Inventor 郭英姝刘斐胡银华张书圣
Owner LINYI UNIVERSITY
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