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Biologicalremediation agent forcadmium pollution and preparation method thereof

A bioremediation agent and cadmium-contaminated technology, applied in biochemical equipment and methods, restoration of contaminated soil, and methods based on microorganisms, can solve the problems of reducing the bioavailability of cadmium, unsatisfactory actual results, and costing a lot of money , to achieve good treatment effect, reduce cadmium toxicity, and low cost

Inactive Publication Date: 2019-04-16
天津天丰泽田生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The physical methods are mainly traditional methods such as soil dumping, alien soil, deep plowing and plowing. Although the effect of this method is obvious, it needs a lot of money and is easy to cause secondary pollution.
The chemical method is mainly to change the physical and chemical properties such as the pH value of the soil by adding chemical reagents and improvers to the soil, so that the cadmium in the soil undergoes redox reactions, thereby reducing the bioavailability of cadmium, but this method is easy to cause cadmium to regenerate. activation, and only for less heavily polluted areas
The biological method mainly uses the metabolic activities of microorganisms or animals and plants to reduce the cadmium content in the soil by means of adsorption and enrichment, but the operation of this method is complicated and the actual effect is not ideal.

Method used

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  • Biologicalremediation agent forcadmium pollution and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Preparation of compound microbial agent:

[0025] 1) Prepare nutrient agar medium, including beef extract 4g / L, egg white 10g / L, sodium chloride 5g / L, and agar 20g / L; inoculate Bacillus thuringiensis on nutrient agar plate, and culture it at 28°C for 8- After 12 hours, wash the activated Bacillus thuringiensis from the nutrient agar plate with sterile physiological saline to make a bacterial suspension; inoculate the bacterial suspension on the seed medium, and cultivate it at 28°C for 8-12 hours to obtain seeds The seed medium contains: sucrose 5g / L, soybean powder 10g / L, potassium dihydrogen phosphate 1g / L, zinc sulfate 0.1g / L; then the seed liquid is inoculated into the fermentation medium for fermentation to obtain Bacillus thuringiensis fermentation Fermentation medium includes: glucose 20g / L, soybean meal powder 10g / L, ammonium sulfate 10g / L, dipotassium hydrogen phosphate 2g / L, potassium dihydrogen phosphate 1g / L, sodium chloride 1g / L, magnesium sulfate 0.5 g...

Embodiment 2

[0031] According to the preparation method of the bacterial liquid in Example 1, the prepared Bacillus thuringiensis bacterial liquid, Aspergillus versicolor bacterial liquid, and Sporotrichum thermophilic bacterial liquid were mixed according to the following proportions to make a composite bacterial liquid, Aspergillus versicolor 10%, thermophilic Disposalis 3%, Bacillus thuringiensis 2%; other biological raw materials include: 10% lumbrokinase, 25% lumbrokinase, 15% shell powder, 25% diatomaceous earth, 10% discarded roots, stems and leaves of cauliflower and cabbage.

Embodiment 3

[0033] According to the preparation method of the bacterial liquid in Example 1, the prepared Bacillus thuringiensis bacterial liquid, Aspergillus versicolor bacterial liquid, and Sporotrichum thermophilic bacterial liquid were mixed according to the following proportions to make a composite bacterial liquid, Aspergillus versicolor 5%, thermophilic Disposalis 5%, Bacillus thuringiensis 5%; other biological raw materials include: 12% lumbrokinase, 22% lumbrokinase, 16% shell powder, 20% diatomaceous earth, 15% discarded roots, stems and leaves of cauliflower and cabbage.

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Abstract

The invention belongs to the technical field of soil heavy metal pollution treatment, and particularly relates to a biological remediation agent for cadmium pollution and a preparation method thereof.The invention provides a biological remediation method aiming at cadmium pollution, which comprises the following steps: a compound bacterial solution is selected in the method, a cell wall containing chitin, chitosan and shell polysaccharide as main components in aspergillus versicolor can adsorb cadmium ions so as to reduce cadmium toxicity; bacillus thuringiensis can be used as microbial low-toxicity insecticide, and thermophilus thermophilus can improve soil and increase soil fertility; in addition, the lumbrokinase and earthworm dung used in the method can absorb the cadmium in the soil,thereby reducing the proportion of the cadmium in the soil and achieving the purpose of remedying the polluted soil; the method also adds waste roots, stems and leaves of diatomite, shell powder, cabbage and cauliflower which can effectively absorb high-concentration cadmium as an adsorbent, and are low in cost and easy to obtain.

Description

technical field [0001] The invention belongs to the technical field of soil heavy metal pollution control, and in particular relates to a bioremediation agent for cadmium pollution and a preparation method. Background technique [0002] Cadmium pollutes the soil in two ways. One is that the cadmium in the industrial waste gas diffuses with the wind and accumulates in the soil around the factory through natural settlement. The other is that the cadmium-containing industrial wastewater irrigates the farmland, making the soil Contaminated by cadmium. Soil environmental pollution caused by excessive soil cadmium ion content will affect the original biological population, population structure, biological activity and food chain structure in the environment, thus endangering the original ecological balance. When the soil is polluted by cadmium, some sensitive biological species in the soil environment will disappear, the activity of environmental microorganisms beneficial to plan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/20B09C1/10C12R1/07C12R1/645
CPCB09C1/10B09C1/105C12N1/14C12N1/20
Inventor 邢军高雪燕张见
Owner 天津天丰泽田生物科技有限公司
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