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Lactobacillus reuteri capable of alleviating allergic asthma and application thereof

A technology of Lactobacillus reuteri and allergic asthma, applied in the field of Lactobacillus reuteri, can solve the problems of recurrent disease, poor effect, and unclear relationship between effects, etc.

Active Publication Date: 2019-04-16
无锡特殊食品与营养健康研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, since most bronchodilator drugs and anti-inflammatory drugs need to be administered by intravenous injection or inhalation, the patient's compliance with these drugs is poor, which leads to repeated attacks of the patient's disease, and over time it will cause the patient's Severe complications such as emphysema and pulmonary heart disease
[0009] Although probiotic preparations are relatively safe and healthy, the currently discovered probiotics such as Lactobacillus adderiformis, Lactobacillus rhamnosus and Bifidobacterium animalis that have been found to relieve the symptoms of allergic asthma may have poor or unclear effects. Therefore, most probiotic preparations on the market also have the defect of slow effect, or have the defect of unclear action relationship
[0010] Therefore, there is an urgent need to find a probiotic that is highly specific to allergic asthma and has a strong ability to relieve allergic asthma, so as to solve the problems that the existing probiotic preparations for allergic asthma are slow to take effect and the relationship between their effects is not clear

Method used

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  • Lactobacillus reuteri capable of alleviating allergic asthma and application thereof
  • Lactobacillus reuteri capable of alleviating allergic asthma and application thereof
  • Lactobacillus reuteri capable of alleviating allergic asthma and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1: Screening and identification of Lactobacillus reuteri

[0061] Specific steps are as follows:

[0062] 1. Screening

[0063] Taking human feces from Dali, Yunnan as a sample, the sample is pretreated and stored in a refrigerator at -80°C in about 20% glycerol. After thawing, the sample is mixed and 0.5mL is added to 4.5mL to contain Make gradient dilutions of 0.05% cysteine ​​in 0.9% normal saline, choose appropriate gradient dilutions and spread them on MRS solid medium supplemented with 0.05% cysteine, and incubate at 37°C for 48 hours. Pick typical colonies to The MRS solid medium was streaked and purified, a single colony was picked and transferred to MRS liquid medium (containing 0.05% cysteine) for enrichment, and preserved with 30% glycerol to obtain the strain CCFM1040, strain R1 and strain R2.

[0064] 2. Identification

[0065] The genome of CCFM1040, R1, R2 was extracted, and the 16S rDNA of CCFM1040, R1, R2 was amplified and sequenced (by Huada Gene Techn...

Embodiment 2

[0066] Example 2: Cultivation of Lactobacillus reuteri

[0067] Specific steps are as follows:

[0068] After inserting Lactobacillus reuteri CCFM1040 into MRS solid medium (containing 0.05% cysteine) and culturing at 37°C for 48 hours, the colonies were observed and the bacteria were observed under a microscope. The colonies are milky white, irregular, round, convex, and smooth, and the bacteria are slightly irregular in shape, with rounded ends of Campylobacter, usually single, paired and small clusters.

[0069] Lactobacillus reuteri CCFM1040 was inserted into MRS liquid medium (containing 0.05% cysteine) and cultured at 37°C for 18 hours, then transferred to fresh MRS liquid medium (containing 0.05% cysteine) ), cultured under the same conditions for 18h, centrifuged at 6000g for 15min, washed with 0.9% saline and centrifuged again at 6000g for 10min to obtain the bacteria, resuspended in 20% sucrose solution, frozen at -80℃ for later use.

Embodiment 3

[0070] Example 3: Effect of Lactobacillus reuteri on HDM-IgG1 in serum of mice with allergic asthma

[0071] Specific steps are as follows:

[0072] The 6-week-old SPF-grade BALB / c female mice were divided into 5 groups, namely the normal group, the model group and the experimental group. Among them, the experimental group included the Lactobacillus reuteri CCFM1040 prevention group by intragastric administration of Lactobacillus reuteri CCFM1040 , Lactobacillus reuteri R1 prevention group by gavage of Lactobacillus reuteri R1 and Lactobacillus reuteri R2 prevention group by gavage of Lactobacillus reuteri R2, 6 in each group, raised in the Experimental Animal Center of Jiangnan University, Ordinary feed feeding, constant temperature 21-26℃, humidity 40-70%, noise less than or equal to 60dB, animal illumination 15-20LX (all animal experiment procedures are reviewed and approved by the Animal Welfare and Ethics Management Committee of Jiangnan University).

[0073] The experiment las...

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Abstract

The invention discloses lactobacillus reuteri capable of alleviating allergic asthma and application thereof and belongs to the technical field of medicines. The lactobacillus reuteri has an effect ofrelieving allergic asthma, which is embodied by: (1) significantly reducing lung inflammation in mice with allergic asthma; (2) significantly inhibiting the generation of dust mite specific immunoglobulin IgG1 in serum of allergic asthma mice; (3) significantly reduced the contents of IL-5 and IL-13 in alveolar lavage fluid of mice with allergic asthma; (4) significantly reducing the content of IL-17A in alveolar lavage fluid of mice with allergic asthma, and thus the lactobacillus reuteri has great application prospects in the preparation of products for preventing and / or treating allergic asthma.

Description

Technical field [0001] The invention relates to a strain of Lactobacillus reuteri that can relieve allergic asthma and its application, and belongs to the field of microbial technology and medical technology. Background technique [0002] Allergic asthma is a chronic airway inflammation involving a variety of cells, especially mast cells, eosinophils and T lymphocytes. In susceptible persons, such inflammation can cause recurrent symptoms such as wheezing, shortness of breath, chest tightness and / or coughing, which can lead to complications such as respiratory arrest and respiratory failure, and endanger the lives of patients. [0003] According to statistics, in the past ten years, there have been about 100 million allergic asthma patients in the world; the prevalence and mortality of allergic asthma in the United States, the United Kingdom, Australia, New Zealand and other countries have gradually increased; there are about 1,000 in China. The average prevalence of allergic asth...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L33/135A61K35/747A61P11/06A61P37/08C12R1/225
CPCA61P11/06A61P37/08A23L33/135A23V2002/00A61K2035/115C12R2001/225C12N1/205A23V2200/314A23V2400/173
Inventor 陈卫陆文伟李凌智翟齐啸张秋香王刚赵建新张灏
Owner 无锡特殊食品与营养健康研究院有限公司
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