Application of diclofenac sodium in the preparation of fungicides for preventing and treating plant diseases caused by plant pathogenic bacteria
A technology of diclofenac sodium and plant pathogenic bacteria is applied in the field of agricultural biology and achieves the effects of broad research and market application prospects, poor drug resistance and good inhibitory activity
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[0036] 1. Experimental materials
[0037] Diclofenac sodium was purchased from Sigma. Weigh 3.18g of diclofenac sodium into 100ml of dimethyl sulfoxide to prepare a 0.1M mother solution, filter and sterilize and store at 4°C until use.
[0038] In this experiment, the plant pathogenic bacteria were stored in a refrigerator at 4°C, and the medium used was potato dextrose medium (PDA for short).
[0039] PDA medium formula: potato (peeled) 200g, glucose 20g, agar 15g, tap water 1000ml, natural pH.
[0040] Preparation method: wash and peel the potatoes, weigh 200g and cut into small pieces, add water and boil (boil for 15-20 minutes), filter in a beaker with eight layers of gauze, add 15-20g of agar according to the needs of the experiment, add 20g of glucose, Stir evenly, fully dissolve, then cool slightly to make up water to 1000ml, dispense and sterilize at 121°C for 20 minutes, cool and set aside.
[0041] 2. Experimental method
[0042] using the growth rate method.
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