Application of sodium diethyldithiocarbamate in the preparation of fungicides for preventing and treating plant diseases caused by plant pathogenic bacteria
A technology of sodium diethyldithiocarbamate and plant pathogenic bacteria is applied in the application field of fungicides to achieve the effects of ensuring high quality, broad research and market application prospects, and poor drug resistance
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[0036] 1. Experimental materials
[0037] Sodium diethyldithiocarbamate was purchased from Sigma. Weigh 2.25g of sodium diethyldithiocarbamate in 100ml of distilled water to prepare a 0.1M mother liquor, filter and sterilize and store at 4°C until use.
[0038] In this experiment, the plant pathogenic bacteria were stored in a refrigerator at 4°C, and the medium used was potato dextrose medium (PDA for short).
[0039] PDA medium formula: potato (peeled) 200g, glucose 20g, agar 15g, tap water 1000ml, natural pH.
[0040] Preparation method: wash and peel the potatoes, weigh 200g and cut into small pieces, add water and boil (boil for 15-20 minutes), filter in a beaker with eight layers of gauze, add 15-20g of agar according to the needs of the experiment, add 20g of glucose, Stir evenly, fully dissolve, then cool slightly to make up water to 1000ml, dispense and sterilize at 121°C for 20 minutes, cool and set aside.
[0041] 2. Experimental method
[0042] using the growth...
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