Applications of fluvoxamine maleate in preparation of bactericide for preventing and treating plant diseases caused by phytopathogens
A technology of fluvoxamine maleate and plant pathogenic bacteria, which is applied in the field of bactericidal active compounds to achieve the effects of non-target organisms and human and animal safety, high quality assurance, and broad prospects for research and market application
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[0041] 1. Experimental materials
[0042] Fluvoxamine maleate was purchased from Sigma. Weigh 4.3441g of fluvoxamine maleate in 100ml of distilled water to prepare a 0.1M mother solution, filter and sterilize and store at 4°C until use.
[0043] In this experiment, the plant pathogenic bacteria were stored in a refrigerator at 4°C, and the medium used was potato dextrose medium (PDA for short).
[0044] PDA medium formula: potato (peeled) 200g, glucose 20g, agar 15g, tap water 1000ml, natural pH
[0045] Preparation method: wash and peel the potatoes, weigh 200g and cut into small pieces, add water and boil (boil for 15-20 minutes), filter in a beaker with eight layers of gauze, add 15-20g of agar according to the needs of the experiment, add 20g of glucose, Stir evenly, fully dissolve, then cool slightly to make up water to 1000ml, dispense and sterilize at 121°C for 20 minutes, cool and set aside.
[0046] 2. Experimental method
[0047] using the growth rate method.
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