Use of butyroxylic acid in the preparation of fungicides for preventing and treating plant diseases caused by plant pathogenic bacteria
A technology of phytopathogenic bacteria and butadiene hydroxy acid, which is applied in the field of fungicides for plant diseases, can solve the problems of reports on the inhibitory effect of butyl hydroxy acid
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[0035] 1. Experimental materials
[0036] Butyric acid is a finished product purchased from Fluka. Weigh 4.47g of butylbenzene hydroxy acid in 20ml of dimethyl sulfoxide to prepare a 1M mother liquor, filter sterilize and store at 4°C until use.
[0037] The plant pathogens used in this experiment are strains stored at 4°C in the laboratory, and the medium used is potato medium (PDA for short).
[0038] PDA medium formula: 200g potato (peeled), 20g glucose, 15g agar, 1000ml tap water, natural pH.
[0039] Preparation method: wash and peel the potatoes, weigh 200g and cut into small pieces, add water to boil (boil for 20-30 minutes, can be pierced by a glass rod), filter in a beaker with eight layers of gauze, add according to the needs of the experiment 15-20g agar, add 20g glucose, stir evenly, fully dissolve and cool to make up to 1000ml of water. After aliquoting, sterilize at 121°C for 20 minutes, then cool for use.
[0040] 2. Experimental method
[0041] Use the growth rate metho...
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