Application of benzydamine hydrochloride in the preparation of fungicides for preventing and treating plant diseases caused by plant pathogenic bacteria
A technology of benzydamine hydrochloride and plant pathogenic bacteria, applied in the field of bactericidal active compounds, to achieve the effect of broad research and market application prospects, poor drug resistance, and high quality
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[0029] 1. Experimental materials
[0030] 1. Benzydamine hydrochloride was purchased from Sigma Company and stored at 4°C until use.
[0031] 2. In this experiment, the plant pathogenic bacteria were stored in a refrigerator at 4°C, and the medium was potato dextrose medium (referred to as PDA). PDA formula: 200 g potato (potato), 20 g glucose, 20 g agar, 1000 ml distilled water, natural pH. Preparation method: Wash and peel the potatoes, weigh 200 g and cut into small pieces, add 1000 g of distilled water to boil for 20 min, then filter it into a large beaker with four layers of gauze, add 20 g of agar, add 20 g of glucose, and stir evenly according to the needs of the experiment , add water to make up to 1000 ml, sterilize at 121°C for 20 minutes after aliquoting, and cool down for later use.
[0032] 3. Yeast extract peptone glucose medium (YPD for short) was used to measure the spore germination and germ tube elongation of plant pathogenic bacteria in this experiment. Y...
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