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An automated transport and posture adjustment system for larval zebrafish

A technology of automatic delivery and posture system, which is applied in the fields of animal restraint equipment, medical science, veterinary equipment, etc., can solve the problems of long time consumption and low success rate, and achieve the goal of reducing experiment time, reducing injury and improving injection efficiency Effect

Active Publication Date: 2019-10-08
哈尔滨工业大学人工智能研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problems of long time-consuming and low success rate in the existing injection of juvenile zebrafish, and to provide a system for automatic delivery and posture adjustment of juvenile zebrafish

Method used

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  • An automated transport and posture adjustment system for larval zebrafish
  • An automated transport and posture adjustment system for larval zebrafish
  • An automated transport and posture adjustment system for larval zebrafish

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0028] Specific implementation mode one: the following combination figure 1 and figure 2 Describe this embodiment, the automatic transportation and posture adjustment system of zebrafish juveniles described in this embodiment, the automatic transportation and posture adjustment system includes a console, a mechanical arm rotation structure 11 and a microfluidic device 4;

[0029] The console includes a microscope 1, a camera 2, a camera fixing device 3, a syringe pump 5, a base 6, a petri dish 7, an LED light source 8, a petri dish fixing device 9 and a lifting platform 10;

[0030] The rotating structure 11 of the mechanical arm is installed on the lifting platform 10, and the rotating structure 11 of the mechanical arm is equipped with a suction needle 11-1. -1 spin;

[0031] The petri dish 7 is fixedly installed on the platform of the base 6 through the petri dish fixing device 9, the micro flow channel device 4 is installed in the petri dish 7, the microscope 1 is insta...

specific Embodiment approach 2

[0037] Specific implementation mode two: the following combination image 3 Describe this embodiment, this embodiment will further explain Embodiment 1, the mechanical arm rotation structure 11 includes a suction needle 11-1, a syringe holder 11-2, a left locking nut 11-3, and a left compression nut 11 -4, right lock nut 11-5, right compression nut 11-6, first deep groove ball bearing 11-7, second deep groove ball bearing 11-8, third deep groove ball bearing 11-9, bearing Seat 11-10, motor 11-11 and synchronous belt 11-12;

[0038] The right locking nut 11-5 and the right compression nut 11-6 are set on the suction needle 11-1, the left locking nut 11-3 and the left compression nut 11-4 are on the syringe holder 11-2, The suction needle 11-1 is installed in the syringe holder 11-2, and the suction needle 11-1 and the syringe holder 11-2 pass through the left compression nut 11-4, the left lock nut 11- 3. The right lock nut 11-5 and the right compression nut 11-6 are fixed, a...

specific Embodiment approach 3

[0042] Specific embodiment three: this embodiment is described further to embodiment one, and camera 2 is connected host computer, and camera 2 detects the attitude of zebrafish larvae in the first observation area 4-12 and the second observation area 4-13, and then The posture of zebrafish larvae was captured and transmitted to the host computer.

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Abstract

The invention discloses an automatic conveying and posture adjusting system for zebra juvenile fishes, and belongs to the field of micro-injection operation. The aim is to solve the problems that in the prior art, when the zebra juvenile fishes are injected, consumed time is long, and the success rate is low. A mechanical arm rotary structure is installed on a lifting table and provided with a sucking-holding needle; a culture dish is fixedly installed on a platform of a base through a culture dish fixing device, a micro flow channel device is installed in the culture dish, a microscope is installed on the platform of the base and located over the micro flow channel device, and a camera is installed on the platform of the base through a camera fixing device; a nonlinear micro flow channelis formed between an upper plate and a lower plate, a micro flow channel head segment, a micro flow channel middle segment and a micro flow channel tail segment are distributed between a fish feedinginlet and a flow channel outlet, the micro flow channel middle segment is provided with a micro flow channel branch, and a second micro flow channel branch is arranged at the boundary of the micro flow channel middle segment and the micro flow channel tail segment. The automatic conveying and posture adjusting system is used for conducting microscopy injection operation on the zebra juvenile fishes.

Description

technical field [0001] The invention relates to an automatic conveying and attitude adjustment system for juvenile zebrafish, which belongs to the field of microinjection operation for juvenile zebrafish. Background technique [0002] As a model animal, zebrafish is playing an increasingly important role in the research fields of life science, health science and environmental science. Its genome sequence has more than 70% similarity with human beings, and it is known as the "water mouse". Zebrafish has biological characteristics such as large egg production, rapid development, transparent embryos, in vitro fertilization and in vitro development, allowing researchers to carry out various experiments more efficiently (Genetic Hereditas (Beijing) August 2018, 40(8) :683-692). [0003] Due to the tiny size of juvenile zebrafish (about 3mm long and 0.5mm wide), there are problems such as long time-consuming and low success rate when manually injecting juvenile fish. Contents o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61D3/00
Inventor 佟明斯杨学博张格非庄松霖马奇智林伟阳高会军
Owner 哈尔滨工业大学人工智能研究院有限公司
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