Application of Liquor saponin r in the preparation of hematopoietic stem cell mobilization agent

A technology of hematopoietic stem cells and mobilizing agent, applied in the field of bioengineering, can solve the problems of short duration of action and high price

Active Publication Date: 2020-02-11
广州沙艾生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Granulocyte colony-stimulating factor is one of the most commonly used hematopoietic stem cell mobilizers, but its action time is short and the price is high. In addition, about 1 / 3 of patients cannot mobilize enough hematopoietic stem cells

Method used

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  • Application of Liquor saponin r in the preparation of hematopoietic stem cell mobilization agent
  • Application of Liquor saponin r in the preparation of hematopoietic stem cell mobilization agent
  • Application of Liquor saponin r in the preparation of hematopoietic stem cell mobilization agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] An agent for mobilizing hematopoietic stem cells, which includes: Liquor saponin R.

[0029] It may further comprise dexamethasone, HS6101.

[0030] The research method of this embodiment:

[0031] Materials: 90 SPF grade C57BL / 6 mice, 8-12 weeks old, half male and half male, weighing 18-24 g, were purchased from Shanghai Experimental Animal Center, Chinese Academy of Sciences. HS6101 was purchased from Zhejiang Hisun Pharmaceutical Co., Ltd. Ficoll-Hypague, dexamethasone, and methylcellulose were purchased from Sigma; Ficoll-Paque Plus lymphocyte separation medium was purchased from GE; bovine serum albumin, fetal bovine serum, glutamine, penicillin, and streptomycin were purchased from From Gibco; IMDM medium was purchased from HyClone.

[0032] Method of administration in groups:

[0033] 90 C57BL / 6 mice were randomly divided into 6 groups: control group, HS6101 group, dexamethasone group, distillery saponin R group, winegrassaponin R + dexamethasone group, wineg...

Embodiment 2

[0035] Peripheral blood hematopoietic stem cell collection Blood was collected from the orbital venous plexus of mice, centrifuged with Ficoll-Hypague density gradient, and the obtained peripheral blood mononuclear cells were washed twice with PBS to make a suspension.

[0036] Collection of bone marrow hematopoietic stem cells Immediately after peripheral blood was collected, the mice were sacrificed by cervical dislocation, and then sterilized with 75% ethanol for 1 min, the bone marrow cells were flushed out with IMDM, blown into a cell suspension, and the agglutinated matter was filtered out with a filter with a pore size of 70 μm , Ficoll-Paque Plus Lymphocyte Separation Medium to separate mononuclear cells. Centrifuge at 1000×g for 8 min at 4°C to make a mononuclear cell suspension. Take 1×10 9 L -1 The cells were inoculated in the IMDM medium (containing penicillin and streptomycin) with a volume fraction of 15% fetal bovine serum, placed in an incubator with a volume...

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Abstract

The invention discloses a hematopoietic stem cell mobilization agent. The hematopoietic stem cell mobilization agent is prepared from conyza japonica saponin R, and can be further prepared from dexamethasone, HS6101 and the like. CD34 is selectively expressed on the surfaces of early hematopoietic stem / progenitor cells and is the main marker for hematopoietic stem cell isolation and purification.If the number of hematopoietic stem cells in peripheral blood is increased, the number of white blood cells can also be increased. Therefore, the number of white blood cells in the peripheral blood can serve as an index reflecting the content of the hematopoietic stem cells in the peripheral blood. It is found through experiments that injection of the conyza japonica saponin R or dexamethasone cansignificantly increase the content of the white blood cells in the peripheral blood, and the combination of drugs had a synergistic effect. A brand-new hematopoietic cell mobilization agent is found,the toxic and side effects are low, the action effect is good, and the agent has a remarkable synergistic effect with other drugs.

Description

technical field [0001] The invention relates to a hematopoietic stem cell mobilization agent and its application, belonging to the technical field of bioengineering. Background technique [0002] Hematopoietic stem cells are adult stem cells in the blood system. They are immature and are the origin of hematopoietic cells and immune cells. They can differentiate into red blood cells, white blood cells and platelets, and can also differentiate into cells of various tissues and organs across systems. They have self-renewal, Multilineage differentiation and homing (ie directed migration to hematopoietic tissues and organs) potential. Hematopoietic stem cells are multifunctional stem cells, also known as "universal cells", which are the ancestor cells of the human body. [0003] Hematopoietic stem cell transplantation refers to the transplantation of allogeneic or autologous hematopoietic stem cells into the recipient's body to undertake corresponding functions, including the re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/704A61K31/573A61K38/19A61P7/00
Inventor 徐智峰张新
Owner 广州沙艾生物科技有限公司
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