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Gucy1a1 gene-specific SNP marker, detection method and application of litter size traits of Hetian Qiaoda red sheep

A litter size and marking technology, applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc.

Active Publication Date: 2022-03-11
XINJIANG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At home and abroad, there are few studies on the SNP markers of sheep litter size, and most of them focus on the research on the functional genes of BMP15, BMPR-IB, and GDF9; among them, there is no report on the SNP markers affecting the litter size of Hetian Qiaoda red sheep

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  • Gucy1a1 gene-specific SNP marker, detection method and application of litter size traits of Hetian Qiaoda red sheep
  • Gucy1a1 gene-specific SNP marker, detection method and application of litter size traits of Hetian Qiaoda red sheep
  • Gucy1a1 gene-specific SNP marker, detection method and application of litter size traits of Hetian Qiaoda red sheep

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Embodiment 1

[0036] Reagents and equipment: magnetic stand (Life Technologies DynaMagTM-2);

[0037] Centrifuge (eppendorf Centrifuge 5424); Douglas Scientific NEXAR; Douglas Scientific Soellex; Douglas Scientific ARRAY; all provided by Biomike Biological Co., Ltd.;

[0038] Mix: KASP 2×Master Mix KBS-1016-011 1536 Formulation V4.0 (provided by LGC).

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Abstract

The invention discloses a GUCY1A1 gene-specific SNP marker, a detection method for litter size traits of Hetian Qiaoda red sheep and its application, and relates to the field of molecular breeding. The SNP marker of the present invention is derived from the sheep GUCY1A1 gene, the SNP marker is located at position 43266624 on chromosome 17, the original base is C, and the variant base is T; the nucleotide sequence of the SNP marker is SEQ ID NO.1. The litter size of individuals with CC genotype at this SNP site was significantly higher than that of TT genotype. The nucleotide sequence of the primer of the present invention is SEQ ID NO.2-4. The present invention applies the above-mentioned SNP markers, primers and kits to the detection of lambing traits of Hetian sheep, and determines how the lambing traits of the Hetian sheep to be tested are determined by detecting the SNP marker genotype; the above-mentioned SNPs, primers, kits and detection methods of the present invention The method can be used in breeding to screen out varieties with more litters.

Description

technical field [0001] The invention relates to the technical field of molecular breeding, in particular to a GUCY1A1 gene-specific SNP marker, a detection method for litter size traits of Hetian Qiaoda red sheep and its application. Background technique [0002] As one of the earliest livestock domesticated and raised by humans, sheep provide humans with abundant livestock products and play an important role in animal husbandry. Xinjiang's complex topography, vast geographical area, and diverse ecological environment make it one of the regions with the richest domestic sheep breed resources in my country. The main representative breeds formed after long-term selection and breeding by local people in Xinjiang. Among them, Hotan Qiaoda Red Sheep is also an important breed of mutton sheep. It is mainly distributed in Pishan County, Hotan Prefecture, Xinjiang. It has strong resistance to stress and disease, and is extremely resistant to rough Breeding and breeding, it has stro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11
Inventor 刘武军马海玉阿布力孜·吾斯曼决肯·阿尼瓦什方超刘玲玲王琼曹行吕士鹏于茜
Owner XINJIANG AGRI UNIV