Method for predicting influences of alfalfa flavonoid on weaned pig colon microflora and application of alfalfa flavonoid

A technology of alfalfa flavonoids and weaned piglets is applied in the direction of determination/inspection of microorganisms, biochemical equipment and methods, applications, etc., and can solve problems such as unreported alfalfa flavonoids.

Inactive Publication Date: 2019-05-10
HENAN AGRICULTURAL UNIVERSITY
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  • Method for predicting influences of alfalfa flavonoid on weaned pig colon microflora and application of alfalfa flavonoid
  • Method for predicting influences of alfalfa flavonoid on weaned pig colon microflora and application of alfalfa flavonoid
  • Method for predicting influences of alfalfa flavonoid on weaned pig colon microflora and application of alfalfa flavonoid

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Embodiment Construction

[0025] The technical solutions of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0026] 1. Test material

[0027] Alfalfa flavonoids are provided by Shaanxi Lvqing Bioengineering Co., Ltd., which is a yellow powder with a total flavonoid content of 50%.

[0028] 2. Assay main reagents

[0029] Medical saline, alcohol, distilled water, 10% tartaric acid aqueous solution, liquid nitrogen, detection immunity and antioxidant kit (Nanjing Jiancheng Bioengineering Institute)

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Abstract

The invention relates to the field of application of alfalfa flavonoid, in particular to a method for predicting the influences of the alfalfa flavonoid on weaned pig colon microflora and the application of the alfalfa flavonoid. Weaned pig colons fed with the alfalfa flavonoid with different concentrations are bought from a pig farm; content of the colons fed with the different kinds of alfalfa flavonoid is collected, and DNA of microorganisms in the content of the colons is extracted; the DNA of the extracted colon microorganisms is used as a template, a bacterium 16S universal primer is adopted, and fluorogenic quantitative PCR detection is carried out; fluorogenic quantitative PCR products are collected respectively, a sodium hydroxide solution is used for denaturation, a single-chainDNA segment is generated, and a Miseq library is established; Miseq sequencing is carried out according to the Miseq library, and double-end sequence data obtained after Miseq sequencing is analyzed.The alfalfa flavonoid has a certain influence effect on forming of the colon flora, 0.2% of the alfalfa flavonoid is added, and the influence effect on the forming of the colon flora is obvious.

Description

technical field [0001] The invention relates to the field of application of alfalfa flavonoids, in particular to a method for predicting the influence of alfalfa flavonoids on colon microflora of weaned piglets and improving the application value of alfalfa flavonoids. Background technique [0002] The nutrients in the feed cannot be directly absorbed into the piglets, and must be digested in the digestive tract to break down the macromolecular organic substances into simple, soluble, small molecules that can be absorbed. Intestinal damage in weaned piglets due to underdeveloped digestive system, weaning stress causes subsequent diarrhea, the end result is reduced feed intake, insufficient digestion capacity, not only the health of the piglets is compromised, but also the growth performance of the piglets is significantly reduced , which is a serious challenge to the modern all-in and all-out isolation weaning system, and is also an important factor restricting the developme...

Claims

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Application Information

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IPC IPC(8): C12Q1/689C12Q1/6869C12Q1/04A23K50/60A23K50/30A23K20/121
Inventor 严学兵曲根郭玉霞郭志鹏管用卓张靖雪王苗利刘建宇
Owner HENAN AGRICULTURAL UNIVERSITY
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