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A 3D printed artificial ovary bioscaffold capable of activating primordial follicles, its artificial ovary and its application

A primitive follicle and 3D printing technology, applied in the field of preparation of 3D printed bio-scaffolds, can solve the problems that hormone therapy is not suitable for everyone, cannot replace the effect, and is difficult to give dosage, so as to achieve rich nutrition supply, shorten the activation time, The effect of reducing waiting time

Active Publication Date: 2022-03-25
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it has a certain effect on improving menopausal symptoms, there are also some disadvantages, such as: hormone therapy is not suitable for everyone, and patients with breast, uterine diseases, diabetes, high blood pressure and other diseases should use it with caution; The amount required by each person is also different, and it is difficult for doctors to give the most suitable dose, and excessive hormones in the body can cause uncontrollable side effects such as endometrial cancer, ovarian cancer, or breast cancer; externally administered hormones are artificial Synthesis, no matter in terms of composition or proportion, it can never replace the effect of self-secreted hormones

Method used

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  • A 3D printed artificial ovary bioscaffold capable of activating primordial follicles, its artificial ovary and its application
  • A 3D printed artificial ovary bioscaffold capable of activating primordial follicles, its artificial ovary and its application
  • A 3D printed artificial ovary bioscaffold capable of activating primordial follicles, its artificial ovary and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1 Design and preparation of 3D printed biological scaffolds

[0056] (1) Combining computer aided design (CAD), three-dimensional printing technology is used to construct biological scaffolds. The shape of 3D biological scaffolds can be selected as circle, rectangle, square, ellipse or irregular shape, and the internal structure is connected. Pore; Pore size (R): 400μm; Line stacking angle: 30-120 degrees;

[0057] (2) Prepare a 3D printing bioscaffold printing material, which contains a mixture of biocompatible materials, primordial follicle activators, and primordial follicle development promoters; the biocompatible materials are 3%-5% sodium alginate and 8%- The mass ratio of 10% gelatin, sodium alginate and gelatin is 1:1, and it becomes gelatinous at room temperature after mixing. Add 740Y-P10ug / ml, PA 8uM, PRO 2uM, FSH 100mIU, LH 10mIU, EGF 5ng / ml, ITS (used after 100-fold dilution) in the biocompatible material.

[0058] (3) Printing ovarian scaffolds b...

Embodiment 2

[0060] Example 2 Design and preparation of 3D printed biological scaffolds

[0061] The stent prepared in Example 2 is the same as the method in Example 1, except for the formulation of the printing material in step (2), the formulation of the printing material is carried out according to the proportions listed in the following table.

[0062]

[0063]

[0064] Example 2 Acquisition of primordial follicles

[0065] The 3-day-old female mice were killed by cervical dislocation. After sterilizing the skin, the ovaries were aseptically removed from the back and placed in a separation medium (L-15+10%FBS+100IU / ml penicillin+100ug / ml chain Mycin). The accessory tissue around the ovary was removed under a solid microscope and washed three times in separation medium. The mouse ovaries were then divided into tissue fragments containing primordial follicles by microscopically puncturing the ovaries with insulin metered injection needles.

Embodiment 3

[0066] Example 3 Activation of primordial follicles

[0067] Activators are agonists or inhibitors of signaling pathways related to primordial follicle activation, all of which belong to the prior art and can be purchased from the market.

[0068] Cryopreserved or fresh ovarian cortex containing a large number of primordial follicles was minced, incubated in basal medium containing different combinations of follicle activators for 0.5-6 hours, washed three times with normal saline to remove foreign substances, and then transferred to print A good biological scaffold was transplanted under the renal capsule of an adult female mouse with bilateral ovariectomy.

[0069] The formula of the basal culture medium of the follicle activator is:

[0070]

[0071]

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Abstract

The invention provides a 3D printed biological scaffold capable of activating primitive follicles in the ovarian cortex. Using 3D printing technology, the primordial follicle activator, hormones and growth factors that can promote follicle development are mixed into the scaffold material, printed into shape, and then moved into the ovarian cortex fragments containing primitive follicles. After transplanting back into the body, the cortical fragments can have better health. Scaffold protection and richer nutrient supply, the bio-scaffold degrades slowly in the body, releases follicle activator slowly, and continuously activates primordial follicles. This technology greatly shortens the time of primordial follicle activation in vitro, and provides a new research tool for revealing the mechanism of primordial follicle activation; on the basis of avoiding secondary surgery, it reduces the waiting time of patients, and it also provides clinical treatment for premature ovarian failure and assisted reproductive technology. It provides a new choice; at the same time, after the follicle is activated, it secretes a large amount of hormones to regulate the body's endocrine, providing a new research direction for improving menopausal symptoms and delaying aging.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the preparation of a 3D printed biological scaffold capable of activating primordial follicles in ovarian cortex. Background technique [0002] With the rapid development of my country's social economy, the status of women is getting higher and higher, and at the same time, they are also facing increasing work and psychological pressure, and the reproductive age of women of child-bearing age is constantly delayed; coupled with the increasingly serious environmental pollution, various toxic Harmful substances are getting closer and closer to life, and the combination of social and environmental factors has caused a large number of reproductive health problems; and since the opening of the two-child policy, there have been more and more elderly mothers. Therefore, how to make the women of super-fertile age whose ovarian function has gradually declined to successfully complete the pregna...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/071C12Q1/02A61P15/08A61P35/00
CPCA61L27/40A61L27/54A61P15/08A61P35/00C12Q1/02
Inventor 张键杨雅莉赵华山
Owner SHENZHEN INST OF ADVANCED TECH
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