Photocrosslinking type decellularized valve and preparation method and application thereof

A decellularized valve, photocrosslinking technology, applied in medical science, prosthesis and other directions, can solve the problems of difficult tissue remodeling, cells cannot grow, etc., to achieve difficult calcification, facilitate cell growth, and enhance mechanical properties and stability. Effect

Inactive Publication Date: 2019-05-31
XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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  • Description
  • Claims
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Problems solved by technology

As a result, cells cannot grow on glutaraldehyde-crossli

Method used

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  • Photocrosslinking type decellularized valve and preparation method and application thereof
  • Photocrosslinking type decellularized valve and preparation method and application thereof
  • Photocrosslinking type decellularized valve and preparation method and application thereof

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Example Embodiment

[0035] Example 1

[0036] The preparation of a photocrosslinked decellularized valve includes the following steps:

[0037] Procurement of porcine aortic valve: porcine heart is obtained under clean conditions in the slaughterhouse and placed in cold normal saline containing heparin and quickly transported to the laboratory. Within 1 hour of the death of the pig, cut the porcine aortic valve under aseptic conditions and place it in a high-glycemic DMEM medium containing antibiotics (100U / ml penicillin, 250mg / ml amphotericin B and 100mg / ml streptomycin) at 4°C Mid 12h;

[0038] Decellularization treatment: The decellularization treatment of porcine aortic valve is carried out in a six-well plate. The working volume of each well is 4ml and a maximum of 3 valves can be placed. The specific method of decellularization is: the porcine aortic valve contains 20g / L CHAPS and 2mmol / L TnBP in TRIS-HCI buffer (40mmol / L, pH 7.8), keep shaking at room temperature for 24h, then rinse with steri...

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Abstract

The invention relates to a photocrosslinking type decellularized valve and a preparation method and application thereof. The preparation method comprises the first step of placing the decellularized valve in a riboflavin solution and gently shaking to fully diffuse the riboflavin into the decellularized matrix; the second step of removing the valve processed in the first step from the riboflavin solution, conducting ultraviolet irradiation and crosslinking and cleaning to remove the residual riboflavin in the valve to obtain the photocrosslinking type decellularized valve. The riboflavin and the photocrosslinking are innovatively used for treating and enhancing mechanical properties of the decellularized matrix valve. Results show that the treatment can significantly increase the Young's modulus of the valve and slow down the degradation rate in the body.

Description

technical field [0001] The invention relates to a scaffold material, in particular to a photocross-linked decellularized valve, a preparation method thereof and an application thereof. Background technique [0002] Decellularized matrix is ​​obtained by decellularizing tissues by different decellularization methods. During the process of decellularization, the original shape and biomechanical properties of natural tissues are preserved as much as possible. Decellularized matrix tissue is used as a bionic scaffold material, which is often used in tissue Engineering and regenerative medicine. [0003] However, due to the damage and loss of extracellular matrix such as elastin and proteoglycan in the tissue to varying degrees, the tissue has the disadvantages of decreased mechanical properties and easy degradation after decellularization. The decline in mechanical properties makes the decellularized valve prone to perforation and insufficiency after implantation in the human b...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/50
Inventor 董念国刘春耕乔韡华白鹏史嘉玮吴钰周
Owner XIEHE HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI & TECH UNIV
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