Gene sequencing chip and method
A gene sequencing and chip technology, which is used in biochemical equipment and methods, microbial determination/inspection, biomass post-processing, etc. Monitor and reflect problems such as insufficient quantity and cost reduction to achieve the effect of improving integration and stability, reducing excitation area and reducing cost
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Embodiment 1
[0046] figure 1 A schematic structural diagram of a gene sequencing chip based on tip-enhanced Raman effect in Example 1 of the present invention is shown. Such as figure 1 As shown, the chip includes:
[0047] A substrate 1 , a first optical waveguide 2 , a second optical waveguide 3 , a metal tip 5 , a nanohole 6 , a light source coupling module 7 , and a first signal acquisition module 8 . The first optical waveguide 2 , the second optical waveguide 3 , the metal needle tip 5 , the nanohole 6 , the light source coupling module 7 , and the first signal acquisition module 8 are all formed on the substrate 1 .
[0048] The substrate 1 is a silicon substrate, the first optical waveguide 2, the second optical waveguide 3 and the metal needle point 5 are relatively distributed on the silicon substrate in a T shape, and the nanohole 6 is arranged on the first optical waveguide 2 and the second optical waveguide 3 The area in contact with the metal needle tip 5 runs through the ...
Embodiment 2
[0053] On the basis of the gene sequencing chip based on the tip-enhanced Raman effect described in Example 1, this example adds a second Raman signal acquisition method, such as Figure 4 As shown, the gene sequencing chip also includes a third optical waveguide 4 and a second collector 9, which are formed on the substrate 1, and the third optical waveguide 4 is opposite to the T-shaped first optical waveguide 2 and the second optical waveguide. 3 and the metal needle point 5 form a cross-shaped structure, the nanohole 6 is placed in the middle of the cross, the third optical waveguide 4 has a three-dimensional gradient structure, and one end is opposite to the metal needle point 5, and the second collector 9 is located on the third optical waveguide 4 and has a three-dimensional gradient structure. One end of the structure is opposite to the other end, the third optical waveguide 4 is a MIM structure, and the metal material in the MIM structure is a metal with a strong surfac...
Embodiment 3
[0056] This embodiment proposes a gene sequencing method based on a gene sequencing chip, such as Figure 5 As shown, the methods include:
[0057] S1, the optical field of the optical signal generated by the light source coupler 7 passes through the first optical waveguide 2 and the second optical waveguide 3 for compression enhancement and resonance enhancement to form excitation light.
[0058] Specifically, the optical signal sent by the light source coupler 7 is reflected back and forth by at least one Bragg periodic structure 203 (303) on the first optical waveguide 2 and the second optical waveguide 3, realizing resonance enhancement, and passing through the three-dimensional tapered structure 204 (304) The optical signal light field is further compressed and enhanced to form excitation light.
[0059] S2, controlling the base chain of the gene to be tested to pass through the nanopore 6 in the form of a single strand;
[0060] Because the size of the nanopore 6 is la...
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