Gene sequencing chip and gene sequencing method

Abstract

The invention discloses a gene sequencing chip and a gene sequencing method. The gene sequencing chip comprises a substrate (1), a first optical waveguide (2), a second optical waveguide (3), a metalneedle tip (5), nano-pores (6); the first optical waveguide (2), the second optical waveguide (3) and the metal needle tip (5) are all formed, in T-shaped relative distribution, on the substrate (1);the first optical waveguide (2) and the second optical waveguide (3) are oppositely positioned; the nano-pores (6) are arranged in contact areas of the first optical waveguide (2), the second opticalwaveguide (3) and the metal needle tip (5), and penetrate through the substrate (1); one ends of the first optical waveguide (2) and the second optical waveguide (3) are both three-dimensional taperedstructures; the first optical waveguide (2) and the second optical waveguide (3) are opposite to each other by the ends with the three-dimensional tapered structures; a light source coupler (7) is arranged, on the other end opposite to the end with the three-dimensional tapered structure, on the first optical waveguide (2); and a first signal collector (8) is arranged, on the other end opposite to the end with the three-dimensional tapered structure, on the second optical waveguide (3). The gene sequencing chip and the gene sequencing method improve detection efficiency of base monomers, thereby reducing cost with integration level and stability of the chip system improved; moreover, data volume is reduced so as to have collection rate improved.

Description

technical field [0001] The invention relates to the field of gene sequencing, in particular to a gene sequencing chip and method. Background technique [0002] Nucleotide sequencing technology is one of the key and basic technologies in genomics and even life science research. It is also the main means of obtaining basic biological information data and the driving force behind the development of biological computing and bioinformatics. Since the birth of Sanger sequencing method, sequencing technology has greatly promoted the development of life science and medicine. In the past 10 years, the successful application of high-throughput second-generation DNA sequencing technology has once again promoted the rapid development of the field of medical and life sciences, and has given birth to the emergence of "precision medicine". However, all second-generation DNA sequencing technologies utilize biochemical reactions of DNA polymerase or ligase to determine DNA sequencing indire...

AI Technical Summary

Problems solved by technology

[0003] Since the current DNA sequencing technologies are all indirect sequencing, that is, based on the observation of labeled fluorescence or autofluorescence during the enzymatic biochemical reaction of nucleic acids, such technologies are limited by the fluorescence attenuation caused by "enzyme fatigue" and the optical Due to factors such as the size of the detection device, the continuity of sequence analysis and the number of simultaneous monitoring reactions are insufficient, resulting in short read length or low throughput

At the same time, fluorescence detection technology requires expensive labeling reagents or autofluorescence reaction systems, making it difficult to further reduce costs on the basis that the throughput level has almost reached the limit

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