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A kind of Lactobacillus rhamnosus and its screening method and application

A technology of Lactobacillus rhamnosus and bacterial liquid, which is applied in the field of microbiology, can solve the problems of inability to digest distiller's grains in a short period of time, distiller's grains are prone to mildew and corruption, unfavorable anaerobic digestion, etc., and achieve the effect of inhibiting the growth, reproduction and storage of miscellaneous bacteria Stable, easy-to-operate effects

Active Publication Date: 2021-01-19
中国环境保护集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the requirements of the brewing process, distiller's grains will be produced in a large scale, considering the construction cost and many other issues, the anaerobic digestion process cannot consume the concentrated distiller's grains in a short time. supply, distiller's grains need a period of time to store
At present, the main storage method of distiller's grains is natural stacking, but naturally stacked distiller's grains are prone to mildew and corruption, resulting in serious loss of organic matter and increased ash content; not only that, a series of biochemical reactions during natural storage have changed its structure to a certain extent and components, which is not conducive to the subsequent anaerobic digestion

Method used

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  • A kind of Lactobacillus rhamnosus and its screening method and application
  • A kind of Lactobacillus rhamnosus and its screening method and application
  • A kind of Lactobacillus rhamnosus and its screening method and application

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Experimental program
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Effect test

Embodiment 1

[0017] Embodiment 1: Screening of Lactobacillus rhamnosus strain

[0018] (1) Preparation of MRS separation medium: Weigh 10g of peptone, 10g of beef extract, 5g of yeast extract, 5g of glucose, 5g of sodium acetate, 2g of diammonium citrate, 80mL of Tween, 0.58g of magnesium sulfate, 0.05g of manganese sulfate, Dipotassium hydrogen phosphate 2g, calcium carbonate 20g, add water to 1000mL, pH 6.5, sterilize at 121°C for 30min, set aside.

[0019] (2) Collect the leachate from a distiller's grain storage base in Yaxi Town, Guizhou (any wine tank storage site is acceptable), and the leachate is divided into 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 Concentration gradients were diluted sequentially, 0.05mL was drawn from the dilutions of each concentration, spread evenly on the MRS separation medium plate, and incubated at a constant temperature at 30°C for 24 hours, and the morphology of each colony was checked under the microscope, rod-shaped colonies were picked, and drawn repeate...

Embodiment 2

[0023] Embodiment 2: the preparation of culture medium and inoculum solution

[0024] 1) Preparation of activated medium: Weigh 10g of peptone, 10g of beef extract, 5g of yeast extract, 5g of glucose, 5g of sodium acetate, 2g of diammonium citrate, 80mL of Tween, 0.58g of magnesium sulfate, 0.05g of manganese sulfate, dihydrogen phosphate Potassium 2g, add water to 1000mL, pH 6.5, high temperature sterilization at 121°C for 30min.

[0025] 2) Preparation of fermentation expansion medium: Weigh 5g of peptone, 5g of beef extract, 5g of yeast extract, 10g of glucose, 5g of sodium acetate, 2g of diammonium citrate, 80mL of Tween, 0.58g of magnesium sulfate, 0.05g of manganese sulfate, hydrogen phosphate Dipotassium 2g, add water to pH 6.5, and sterilize at 121°C for 30min.

[0026] 3) Preparation of the inoculum solution: inoculate Lactobacillus rhamnosus into the activation medium and activate the sample for 48 hours to obtain activated strains; inoculate the activated strains i...

Embodiment 3

[0027] Embodiment 3: the application of Lactobacillus rhamnosus

[0028] Option One:

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Abstract

The invention relates to the technical field of microorganisms, and particularly discloses lactobacillus rhamnosus with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.14568. The lactobacillus rhamnosus disclosed by the invention is inoculated into vinasse; carbohydrates and other substances in the vinasse can be used as the basis to perform lactic acid fermentation, lactic acid has very strong sterilization capability, and can effectively inhibit the activity of harmful microorganisms and the rapid decomposition of organic matters, so that the lactobacillus rhamnosus inoculated in the vinasse can well grow in the vinasse storage process, and can inhibit the growth and propagation of infectious microbes such as mould and the like in the vinassestorage process, thereby realizing the maximum maintenance of nutrient components in the vinasse raw material storage process.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a lactobacillus rhamnosus and its screening method and application. Background technique [0002] With the development of liquor technology in our country, the annual output of liquor is gradually increasing, and the output of distiller's grains, which is a by-product of liquor production, is also increasing. The reuse of distiller's grains has become an urgent problem to be solved in the liquor industry. In recent years, the annual output of liquor in my country has reached 5-6 million tons, and the output of distiller's grains has reached 21 million tons. Proper disposal of distiller's grains will directly affect the sustainable development of liquor production enterprises, and it is also related to the promotion and implementation of relevant national grain saving and environmental protection policies. Distiller's grains are rich in amino acids, vitamins and a variety ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02C12P7/56C12R1/225
Inventor 何荣玉徐太平胡兵
Owner 中国环境保护集团有限公司
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