A kind of preparation method of electrochemical aptamer sensor for detecting aflatoxin b1

An aptamer sensor and aflatoxin technology, applied in the direction of material electrochemical variables, etc., to achieve the effect of simple construction process, high sensitivity, and uncomplicated operation

Active Publication Date: 2021-07-09
TIANJIN UNIV OF SCI & TECH
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no method for combining aptamers with sensors for the detection of aflatoxin B1

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of preparation method of electrochemical aptamer sensor for detecting aflatoxin b1

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A preparation method for detecting aflatoxin B1 electrochemical aptamer sensor, the sensor includes a working electrode, a reference electrode and a counter electrode, the working electrode is a glassy carbon electrode, and the reference electrode is saturated potassium chloride glycerin Mercury electrode, counter electrode is platinum column electrode, comprises the following steps:

[0040] 1) Modify mesoporous carbon on the working electrode: Dissolve an appropriate amount of mesoporous carbon in N, N-dimethylformamide aqueous solution, obtain a uniformly dispersed mesoporous carbon suspension by ultrasonication, and modify the mesoporous carbon on the surface of the working electrode to Increase the surface area of ​​the working electrode;

[0041] 2) Modification of gold nanoparticles: immerse the modified working electrode in step 1) into HAuCl 4 and H 2 SO 4 Constant potential deposition in the mixed solution to change the surface properties of the working ele...

Embodiment 2

[0046] A preparation method for detecting aflatoxin B1 electrochemical aptamer sensor, the sensor includes a working electrode, a reference electrode and a counter electrode, the working electrode is a glassy carbon electrode, and the reference electrode is saturated potassium chloride glycerin Mercury electrode, counter electrode is platinum column electrode, comprises the following steps:

[0047] 1) Modifying the working electrode with mesoporous carbon: modifying the surface of the working electrode with mesoporous carbon to increase the surface area of ​​the working electrode; the specific steps are:

[0048] Take 1mg of mesoporous carbon and dissolve it in 2ml of N,N-dimethylformamide aqueous solution (v:v, 1:1), sonicate for 30min to obtain a uniformly dispersed concentration of 0.5mg mL -1 Take the mesoporous carbon suspension, take 2-14 μL of the mesoporous carbon suspension and drop it on the pretreated clean working electrode, and let it dry naturally to obtain the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to view more

Abstract

The present invention provides a preparation method for detecting aflatoxin B1 electrochemical aptamer sensor, comprising the following steps, 1) adding mesoporous carbon to the working electrode; 2) depositing gold nanoparticles at a constant potential to CMK / GCE Modification; 3) Drop AFB1 aptamer modified with a sulfhydryl group on the AuNPs / CMK / GCE electrode; 4) Immerse Apt / AuNPs / CMK / GCE in methylene blue (MB) solution, and adsorb MB to the aptamer chain Get the MB‑Apt / AuNPs / CMK / GCE electrode; 5) Detect the reduction peak current value. The aflatoxin B1 electrochemical aptamer sensor created by the invention can achieve the purpose of quantitative detection of the target object, and the characteristics of fastness and simple operation overcome the disadvantages of complex operation and long detection cycle of large-scale detection instruments.

Description

technical field [0001] The invention belongs to the field of photoelectric materials and photoelectrochemical detection, and in particular relates to a preparation method of an electrochemical aptamer sensor for detecting aflatoxin B1. Background technique [0002] Aflatoxin (Aflatoxin) is a carcinogenic mycotoxin. Aflatoxin has different specific structures and toxicity differences. Among them, AFB1 is the most toxic and carcinogenic, and has been identified as a class I carcinogen by the International Organization for Research on Cancer. When it enters the animal body, it will show strong pro-hepatotropism, causing hepatic hemorrhage, fatty degeneration, bile duct hyperplasia and liver cancer. In addition, it will also cause inflammatory lesions in the front part of the stomach, changes in hematopoietic function and blood components, and immune function. damage to other systems. [0003] Electrochemical sensor is a small and portable analysis device, which is composed of ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/48G01N27/30
Inventor 刘冰赵耀帅吴秋月潘明飞王硕
Owner TIANJIN UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap