A kind of diapause control and storage method of long-banded braconids
A storage method and long-body technology, applied in animal husbandry and other directions, can solve problems such as waste of human, material and financial resources, degradation of bee species, and difficulty in long-distance transportation.
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Embodiment 1
[0034] Embodiment 1, the diapause induction experiment of the long body braconid
[0035] 1. Materials and methods:
[0036] 1.1 Source of tested insects
[0037] The larvae of the Asian corn borer were reared using the standard insect culture technology of Zhou Darong et al. and agar-free semi-artificial diet, and were continuously reared in an artificial climate room with a temperature of (28±0.5)°C, a relative humidity of 70%-80%, and a photoperiod of 16L:8D. Multiple generations.
[0038] The long-bodied Braconidus was collected from the mature larvae of the Asian corn borer parasitized in the field experiment base of Gongzhuling City, Jilin Province, and the third-instar larvae of the Asian corn borer were used as hosts for subculture indoors. After parasitism, culture at temperature (28±0.5)℃, relative humidity 70%-80%, photoperiod 16L:8D in an artificial climate box; after cocooning, culture at temperature (24+0.5)℃, relative humidity 60% -70%, photoperiod 16L:8D in ...
Embodiment 2
[0046] Embodiment 2, the diapause storage and removal experiment of the long body branicus of girdle
[0047] 1. Materials and methods
[0048] 1.1 Source of tested insects
[0049] The feeding method of the Asian corn borer and the long-banded braconid was the same as that of experiment 1
[0050] 1.2 Experimental method
[0051] According to the results of the above example 1, this experiment selects the diapause Asiatic parasitizing the diapause belt long body Braconus in vivo induced under the conditions of light 10L: 14D, humidity 70%-80%, temperature light period 21°C and dark period 15°C Corn borer was used as the test object. Carry out the following treatment respectively with the diapause long body branicus:
[0052] 1) First, treat the diapause Braconidus longiformis at 10°C, 4°C, and -4°C for 15, 30, 60, and 90 days:
[0053] 2) After the treatment time is up, take out the long body of the wasp and place it in an incubator with a temperature of (28±0.5)°C, a li...
Embodiment 3
[0065] Embodiment 3, the impact of low temperature storage on the biology of diapause belt long body Braconis after diapause solution
[0066] 3.1 Experimental method
[0067] Put the diapause-free cocoons of the long-bodied wasp under the storage condition of 4°C into an artificial climate box with a temperature of 24°C, a photoperiod of 16L:8D, and a humidity of 70%-80%, until the long-bodied wasp emerges Afterwards, do the following processing: 1) Insert the long body branicus of the girdle within 24 hours of eclosion into a pair of male and female bees (♀:♂=1:1) in a beekeeping cage of 15cm×15cm×15cm, and feed 20% of them simultaneously. Honey water, after 24 hours of mating, use fresh corn cob slices as a carrier, insert 25 3rd instar larvae of Ostrinia officinalis into each beekeeping cage, and after 24 hours of parasitism, take out Ostrinia officinalis and put them in artificial feed for feeding. After 10 days, the parasitism situation was counted, and each treatment w...
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