Culture solution of universal Treg cells and culture method and application of culture solution

Abstract

The invention relates to a culture solution of universal Treg cells and a culture method and application of the culture solution. The culture method of the culture solution of the universal Treg cellscomprises the following steps: S110, co-culturing CD4+ CD25+ Treg cells and hUC-MSCs according to a certain proportion for 48 h-72 h, and centrifuging to obtain supernatant; S220, preparing peripheral blood PBMC and plasma, inactivating the plasma, and centrifuging for later use; S330, adding cytokine, calcium ionophore, plasma obtained from S220 and a RPMI1640 culture solution into the supernatant obtained from S110 to prepare a Treg cell culture solution; S440, inoculating peripheral blood PBMC into the Treg cell culture solution prepared in S330 according to a certain density, and culturing for 48 h-96 h; S550, collecting cells, wherein the cell viability is greater than 90%, the cell expression of CD4+ CD25+ Foxp3+ Treg is greater than 65%, and CD4+ CD25+ Foxp3+ Helios+ Treg cells account for more than 90% of the cell expression of CD4+ CD25+ Foxp3+ Treg. The problems that Treg cell culture operation is complex and the period is too long are solved, and the problems that the content of CD4+ CD25+ Foxp3+ Helios+ Treg cells in peripheral blood is too low and Treg cell treatment cannot be implemented due to a patient with low quality of autologous Treg cells caused by the problemof diseases of the patient are solved.

Description

technical field [0001] The invention relates to the field of cell technology, in particular to a culture solution of universal Treg cells and a culture method and application thereof. Background technique [0002] Regulatory T cells (Treg) are a group of phenotypically and functionally specific T cell subsets newly discovered in recent years. important role in balance. Treg cells are a double-edged sword for the human immune system and endocrine system. It plays a very important role in maintaining the body's immune tolerance and immune response homeostasis. It can suppress an overly strong immune response through a series of mechanisms, allowing the body to effectively remove antigens or pathogens with minimal self-damage. Controlling the number and expression of Treg cells can help us control the process of disease. Treg cells exert immunosuppressive functions mainly in two ways. The first way is that Treg cells can play an immune regulatory role by secreting heterogen...

AI Technical Summary

Problems solved by technology

[0005] At present, Treg cells used in disease treatment research are mostly isolated and cultured from patients' autologous peripheral blood, but for those patients whose autologous Treg cells are of low quality due to autologous disease problems, Treg cell therapy cannot be performed, so the development of non-antigenic general-purpose Sexual Treg cells hold the key to solving the problem