Method for quickly propagating seedlings of dendrobium officinale Kimura et Migo

Abstract

The invention discloses a method for quickly propagating seedlings of dendrobium officinale Kimura et Migo. The method comprises the following steps that ripe capsules of the dendrobium officinale Kimura et Migo are utilized for preparing a seed suspension liquid, dibble seedling is conducted, and then one-step seedling growing culture is conducted. By means of the method, the purpose that the seeds of the dendrobium officinale Kimura et Migo are directly differentiated, grown and developed into robust seedlings with the length of 8-10 cm and with roots, stems and leaves in an organic matter culture medium in which no hormone is added without transfer. The manual transfer frequency in the seedling growing process is removed, the manual cost and tissue culture seedling contamination rate which is caused by transfer are reduced, the seedling survival period brought by seedling hurting due to transfer is removed, thus the growth period of tissue culture seedlings of the dendrobium officinale Kimura et Migo is shortened, the production steps of the seedlings of the dendrobium officinale Kimura et Migo are simplified to a great extent, and the production cost is reduced; moreover, heritable variation of the tissue culture seedlings of the dendrobium officinale Kimura et Migo caused by hormone addition in the tissue culture process can be avoided, the original characters of the seedlings are kept to the greatest extent, and the method can serve as an important method for production of the tissue culture seedlings of the dendrobium officinale Kimura et Migo.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, in particular to a method for rapidly propagating dendrobium officinale seedlings. Background technique [0002] Dendrobium officinale Kimura et Migo is a herbaceous plant of the genus Dendrobium orchidaceae. Because the seeds are extremely small, the embryos are not fully developed, and there is no endosperm tissue, the germination rate is extremely low in the natural state, and the natural reproductive ability is also weak. Coupled with the increasingly severe damage to the ecological environment, wild Dendrobium candidum resources are on the verge of extinction, and have now been listed as one of the key protected medicinal plants by the state. In order to protect this endangered precious traditional Chinese medicine, researchers at home and abroad have carried out tissue culture and rapid propagation of Dendrobium officinale, seed germination and mycorrhizal propagation research, and have...

AI Technical Summary

Problems solved by technology

However, this process needs to be transferred three times, and the steps are still cumbersome. Multiple bottle changes and transfers not only waste manpower, financial resources, and material resources, but also increase the pollution rate of tissue culture seedlings, resulting in a decrease in the rate of dendrobium candidum tissue culture seedlings coming out of bottles. Reduced, resulting in high seedling production costs

Moreover, the addition of hormones in the medium has certain effects on the growth of Dendrobium candidum tissue culture seedlings, and improper hormone addition may cause the traits and genetic variation of tissue culture seedlings.

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