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Ischemia modified albumin detection kit

A detection kit and albumin technology, applied in the field of medical detection, can solve problems such as low accuracy, poor stability, and influence on experimental reproducibility, so as to improve the stability of opening the bottle, improve the stability of the validity period, and achieve good clinical application value effect

Inactive Publication Date: 2019-07-02
曾宪亮
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The embodiment of the present invention provides an ischemia-modified albumin kit, which aims to solve the problem of low detection efficiency, low accuracy, poor stability and affecting the reproducibility of the experiment, which is not conducive to the promotion of the detection reagent. application problem

Method used

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  • Ischemia modified albumin detection kit
  • Ischemia modified albumin detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A detection kit for ischemic modified albumin, comprising R1 reagent and R2 reagent,

[0026] Wherein, the R1 reagent includes the following components: phosphate buffer (pH=7.2) 0.02mol / L; cobalt chloride solution 5mmol / L; liquid biological preservative Proclin300 0.02g / L; triethanolamine 0.5g / L;

[0027] The R2 reagent includes the following components: phosphate buffer (pH=7.2) 0.05mol / L; dithiothreitol 6mmol / L; surfactant (propylene oxide-ethylene oxide block copolymer F88 and The weight ratio of Tween-20 is 3:5) 0.3g / L; liquid biological preservative Proclin300 0.02g / L.

Embodiment 2

[0029] A detection kit for ischemic modified albumin, comprising R1 reagent and R2 reagent,

[0030] Wherein, the R1 reagent includes the following components: phosphate buffer (pH=7.3) 0.04mol / L; cobalt chloride solution 6mmol / L; liquid biological preservative Proclin300 0.04g / L; triethanolamine 0.8g / L;

[0031] The R2 reagent comprises the following components: phosphate buffer (pH=7.3) 0.08mol / L; dithiothreitol 8mmol / L; surfactant (propylene oxide-ethylene oxide block copolymer F88 and The weight ratio of Tween-20 is 3:4) 0.4g / L; liquid biological preservative Proclin300 0.05g / L.

Embodiment 3

[0033] A detection kit for ischemic modified albumin, characterized in that it includes R1 reagent and R2 reagent,

[0034] Wherein, the R1 reagent includes the following components: phosphate buffer (pH=7.6) 0.06mol / L; cobalt chloride solution 7mmol / L; liquid biological preservative Proclin300 0.05g / L; triethanolamine 1g / L;

[0035] The R2 reagent includes the following components: phosphate buffer (pH=7.6) 0.1mol / L; dithiothreitol 10mmol / L; surfactant (propylene oxide-ethylene oxide block copolymer F88 and The weight ratio of Tween-20 is 1:1) 0.5g / L; liquid biological preservative Proclin300 0.06g / L.

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Abstract

The invention, which is applicable to the technical field of medical detection, provides an ischemia modified albumin detection kit, which comprises an R1 reagent and an R2 reagent. The R1 reagent comprises the following components: 0.02 to 0.1 mol / L of phosphate buffers, 5 to 10mmol / L of a cobalt chloride solution, 0.02 to 0.08g / L of preservatives, and 0.5 to 1.5g / L of stabilizers. The R2 reagentincludes the following components: 0.05 to 0.15 mol / L of phosphate buffers, 6 to 15 mmol / L of dithiothreitol, 0.3 to 0.7 g / L of surfactants, and 0.02 to 0.08 g / L of preservatives. The kit can be applied to detection of the content of ischemia-modified albumin in the human serum; the detection result has high repeatability and high precision; and the highest detection range can reach 200.4 U / mL. The kit has the great linical application value.

Description

technical field [0001] The invention belongs to the technical field of medical detection, in particular to a detection kit for ischemic modified albumin. Background technique [0002] Ischemia-modified albumin (IMA) is a new and ideal marker of ischemia, and it is the first marker of myocardial ischemia approved by the US Food and Drug Administration. Many studies have proved that IMA can sensitively reflect myocardial ischemia, and is of great significance in the early diagnosis, risk stratification, and guidance of treatment of acute coronary syndrome. It is currently an ideal biochemical marker for detecting myocardial ischemia. . [0003] However, the detection methods for ischemia-modified albumin include ultrafiltration, liquid phase assay, mass spectrometry, and nuclear magnetic resonance, etc., but they are not suitable for routine clinical use due to high detection cost or poor stability. At present, the main method for detecting ischemia-modified albumin is the a...

Claims

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Application Information

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IPC IPC(8): G01N21/78
CPCG01N21/78G01N2021/775
Inventor 曾宪亮
Owner 曾宪亮
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