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A kind of Gecko-Paecilomyces cicada bidirectional solid fermentation product and its preparation method and application

A technology of bidirectional solid fermentation and Paecilomyces cicadae is applied in the preparation of drugs for the treatment of liver cancer. In the field of bidirectional solid fermentation products of Gecko-Paecilomyces cicadae, it can solve the problems of inability to prepare high-efficiency preparations, and achieve the improvement of anti-tumor function, enhanced The effect of improving the resistance and anti-HepG2 liver cancer cell activity

Active Publication Date: 2021-09-10
经皮永和医药科技(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the clinical application of traditional Chinese medicine, geckos are mostly used as protozoa, original powder or ultrafine powder, which often has the defect that high-efficiency preparations cannot be prepared due to the large amount of preparation.

Method used

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  • A kind of Gecko-Paecilomyces cicada bidirectional solid fermentation product and its preparation method and application
  • A kind of Gecko-Paecilomyces cicada bidirectional solid fermentation product and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The Gecko-Paecilomyces cicadae two-way solid fermentation product described in this embodiment is prepared according to the following steps:

[0034] (1) Gecko pretreatment: Weigh a number of dried gecko medicinal materials and put them into a 35-37°C constant-temperature blast drying oven for drying treatment for 48 hours, and pulverize them with a pulverizer, pass through a 60-mesh sieve, and the filtered product is the gecko powder used, and set aside ;

[0035] (2) Activation of Paecilomyces cicadae strains: pick an appropriate amount of Paecilomyces cicadae mycelium preserved with an inoculation loop, aseptically inoculate it on the sterilized PDA solid medium, and put it into a constant temperature and humidity culture at 28°C and 80% humidity Cultivate in the box for 5 days to obtain activated thalli; then, pick an appropriate amount of Paecilomyces cicadae mycelium from the PDA solid medium with an inoculation loop, insert it into the sterilized PDA liquid mediu...

Embodiment 2

[0040] The Gecko-Paecilomyces cicadae two-way solid fermentation product described in this embodiment is prepared according to the following steps:

[0041] (1) Gecko pretreatment: Weigh a number of dried gecko medicinal materials and put them into a 35-37°C constant-temperature blast drying oven for drying treatment for 48 hours, and pulverize them with a pulverizer, pass through a 60-mesh sieve, and the filtered product is the gecko powder used, and set aside ;

[0042] (2) Activation of Paecilomyces cicadae strains: pick an appropriate amount of Paecilomyces cicadae hyphae preserved with an inoculation loop, aseptically inoculate it on the sterilized PDA solid medium, and put it into a constant temperature and humidity culture at 25°C and 80% humidity Cultivate in the box for 7 days to obtain activated thalli; then, pick an appropriate amount of Paecilomyces cicadae mycelium from the PDA solid medium with an inoculation loop, insert it into the sterilized PDA liquid medium,...

Embodiment 3

[0047] The Gecko-Paecilomyces cicadae two-way solid fermentation product described in this embodiment is prepared according to the following steps:

[0048] (1) Gecko pretreatment: Weigh a number of dried gecko medicinal materials and put them into a 35-37°C constant-temperature blast drying oven for drying treatment for 48 hours, and pulverize them with a pulverizer, pass through a 60-mesh sieve, and the filtered product is the gecko powder used, and set aside ;

[0049] (2) Activation of Paecilomyces cicadae strains: pick an appropriate amount of Paecilomyces cicadae hyphae preserved with an inoculation loop, aseptically inoculate it on the sterilized PDA solid medium, and put it into a constant temperature and humidity culture at 30°C and 90% humidity Cultivate in the box for 4 days to obtain activated thalli; then, pick an appropriate amount of Paecilomyces cicadae mycelium from the PDA solid medium with an inoculation loop, insert it into the sterilized PDA liquid medium,...

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Abstract

The invention belongs to the technical field of traditional Chinese medicine preparations, and specifically relates to a two-way solid fermented product of Paecilomyces gecko-cidiae, and further discloses its preparation method and its application in the preparation of drugs for treating liver cancer. The gecko-Paecilomyces cicadae two-way solid fermentation product of the present invention is obtained by further fermentation with the Paecilomyces cicadae liquid on the basis of the existing gecko medicinal materials, and utilizes the unique medicinal properties and functional components of the gecko and Paecilomyces cicadae, Improve the anti-tumor function of gecko medicinal materials through the deep-level interaction between the two. The gecko-Paecilomyces cicada bidirectional solid fermentation product of the present invention obtains its active ingredients by adding water and extracting it. The water extract has a significant inhibitory effect on HepG2 liver cancer cells, and its inhibition rate is in the range of 60-80%. The anti-HepG2 liver cancer cell activity of the gecko water extract at the same concentration is greatly improved, and can be used to prepare medicines for treating liver cancer.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine preparations, and specifically relates to a gecko-paecilomyces cicada bidirectional solid fermentation product, and further discloses a preparation method thereof, and its application in the preparation of drugs for treating liver cancer. Background technique [0002] Animal medicine is an important part of traditional Chinese medicine. There are 13 prescriptions in the classic of traditional Chinese medicine "Huangdi Neijing", of which 5 prescriptions are used as medicine with human or animal organs and tissues; , There are also a considerable number of records about animal medicinal materials. For example, the "Compendium of Materia Medica" compiled by Li Shizhen in the Ming Dynasty contained 1892 kinds of medicines, of which 444 were animal medicines, accounting for about 1 / 4 of the total. [0003] Gecko is a kind of animal medicine, and its main active ingredients are prot...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/068A61K35/583A61P35/00
CPCA61K35/583A61K36/068A61K2236/19A61K2236/331A61K2236/51A61P35/00A61K2300/00
Inventor 黄金昶史新元连增林杨芳
Owner 经皮永和医药科技(北京)有限公司
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