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A kind of crocodile serum protein with anti-tumor function and its preparation method

A serum protein and anti-tumor technology, applied in the field of functional food processing, can solve the problems of crocodile serum protein production, research and related function reports, etc., and achieve the goal of improving anti-tumor function, realizing industrial production and controlling growth Effect

Active Publication Date: 2018-04-06
广西盟展鳄鱼科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As for the reptile crocodile, people's understanding of it is mainly limited to the introduction in "Compendium of Materia Medica" by Li Shizhen, a treasure of traditional Chinese medicine. Related literature has reported some functional properties of crocodile blood, such as antibacterial activity and antioxidant activity, but the antitumor effect of crocodile blood There are still few specific studies on the function, and there are no reports on the production, research and related functions of crocodile serum protein

Method used

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  • A kind of crocodile serum protein with anti-tumor function and its preparation method
  • A kind of crocodile serum protein with anti-tumor function and its preparation method

Examples

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Effect test

Embodiment 1

[0026] Example 1 A preparation method of crocodile serum protein with anti-tumor function

[0027] (1) 50 grams of crocodile blood is collected in a container, and 0.4 grams of sodium citrate solution (sodium citrate solution concentration is 25%) is added as an anticoagulant in the container, cooled to 15 ° C, and centrifuged at 10000 g for 5 minutes , collecting the crocodile blood supernatant; the supernatant was freeze-dried to obtain 3.5 grams of crocodile serum powder.

[0028] (2) Take 3.5 grams of crocodile serum powder, mix it with water at a ratio of 12% crocodile serum powder (w / w), stir at 30° C. for 10 minutes, make it fully dissolve, and prepare crocodile serum solution. Then, in an ultrasonic generator, it was treated with ultrasonic waves (frequency of 130kH) for 10 minutes to change the tissue structure of the protein.

[0029] (3) Add 0.0175 grams of flavor protease to the ultrasonically treated crocodile serum powder liquid according to the percentage by we...

Embodiment 2

[0031] Example 2 A preparation method of crocodile serum protein with anti-tumor function

[0032] (1) 100 grams of crocodile blood is collected in a container, add 1.2 grams of sodium citrate solution (sodium citrate solution concentration is 25%) as an anticoagulant in the container, cool to 20 ° C, and centrifuge at 8000 g for 10 minutes , collecting the crocodile blood supernatant; the supernatant was freeze-dried to obtain 7.2 grams of crocodile serum powder.

[0033] (2) Take crocodile serum powder, mix it with water at a ratio of 8% crocodile serum powder (w / w), stir at 20° C. for 10-20 minutes, make it fully dissolve, and prepare crocodile serum solution. Then, in an ultrasonic generator, it was treated with ultrasonic waves (frequency 90kH) for 20 minutes to change the tissue structure of the protein.

[0034] (3) Add 0.0144 grams of flavor protease to the ultrasonically treated crocodile serum powder liquid according to the weight percentage ratio of 0.2% of the cro...

Embodiment 3

[0036] Example 3 A preparation method of crocodile serum protein with anti-tumor function

[0037] (1) 10 grams of crocodile blood are collected in a container, add 0.1 gram of sodium citrate solution (the concentration of sodium citrate solution is 25%) in the container as an anticoagulant, cool to 15°C, and centrifuge at 10000g for 10 minutes , collecting the crocodile blood supernatant; the supernatant was freeze-dried to obtain 0.7 g of crocodile serum powder.

[0038] (2) Take crocodile serum powder, mix it with water at a ratio of 10% crocodile serum powder (w / w), stir at 25° C. for 20 minutes, make it fully dissolve, and prepare crocodile serum solution. Then, in an ultrasonic generator, it was treated with ultrasonic waves (frequency of 130kH) for 15 minutes to change the tissue structure of the protein.

[0039] (3) Add 0.00035 grams of flavor protease to the ultrasonically treated crocodile serum powder liquid according to the weight percentage ratio of 0.5% of the ...

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Abstract

The invention relates to the field of the processing of functional foods, and particularly provides crocodilian serum protein with an antineoplastic function and a preparation method thereof. The preparation method of the crocodilian serum protein comprises the following steps of ultrasonically treating a crocodilian serum solution, adding flavorzyme into the crocodilian serum solution to carry out enzymolysis for obtaining enzymatic hydrolysate, and freeze-drying the enzymatic hydrolysate to obtain the crocodilian serum protein with the antineoplastic function. According to the crocodilian serum protein with the antineoplastic function and the preparation method thereof, the crocodilian serum protein is treated by utilizing an ultrasonic technique; the antineoplastic function of the serum protein can be improved obviously; the growth of a tumor cell is effectively controlled.

Description

technical field [0001] The invention relates to the field of functional food processing, in particular to a crocodile serum protein with anti-tumor function and a preparation method. Background technique [0002] At present, pig and cow blood have been used in the food industry to a certain extent, mainly after the blood is degraded, decolorized, dried, and pulverized to make iron-rich food. There is also some research on the preparation of hydrolyzed protein, hemoglobin, superoxide dismutase, immunoglobulin and other products from blood. Crocodiles are an ancient reptile that survived the Cretaceous Period. From the current functional characteristics and utilization development of animal blood, it can be seen that the utilization of livestock and poultry blood has become increasingly mature. As for the reptile crocodile, people's understanding of it is mainly limited to the introduction in "Compendium of Materia Medica" by Li Shizhen, a treasure of traditional Chinese med...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06
Inventor 陈孙福
Owner 广西盟展鳄鱼科技开发有限公司
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