Method for constructing rodent model for intestinal flora

A technology for intestinal flora and rodents, applied in animal husbandry and other directions, can solve the problems of limited effect, limited effect of antibiotics, side effects, etc., and achieve the effect of short modeling time, remarkable effect and good repeatability

Inactive Publication Date: 2019-08-23
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

However, this method has many disadvantages: (1) the use of antibiotics has limited effect and cannot achieve the purpose of eliminating intestinal microorganisms, and can only reduce the content of intestinal microorganisms, which has a limited effect; (2) antibiotics need to be used for a long time, otherwise it cannot achieve the purpose of reducing intestinal The purpose of microorganisms; (3) long-term use of antibiotics has drug resistance and side effects

Method used

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  • Method for constructing rodent model for intestinal flora
  • Method for constructing rodent model for intestinal flora
  • Method for constructing rodent model for intestinal flora

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Establishment method of rodent model for intestinal flora research:

[0033] (1) 2-month-old C57BL / 6 mice were used as experimental animals, male, 10, body weight 30±2.0g, randomly divided into two groups: control group and model group (5 each); reared in a barrier environment, Feed the basic maintenance feed; the food and water required for the day are sterilized, and they can eat and drink freely;

[0034] Control group: Mice were fed with normal saline, twice a day, 1 mL / time / mouse, with an interval of 4 hours, and were fasted for 2 hours before feeding;

[0035] Model group: Mice were given polyethylene glycol electrolyte powder, twice a day, 1 mL / time / mouse, with an interval of 4 hours, and were fasted for 2 hours before feeding;

[0036] (2) Preparation of Polyethylene Glycol Electrolyte Powder: The preparation method of the used Polyethylene Glycol Electrolyte Powder is by potassium chloride 0.74g, sodium bicarbonate 1.68g, sodium chloride 1.46g, sodium sulf...

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Abstract

The invention discloses a method for constructing a rodent model for intestinal flora, and relates to the technical field of research on the intestinal flora. According to the method, a mouse is usedas an experimental object, and is fed with polyethylene glycol electrolyte powder through gavage to obtain the rodent model for the research on the intestinal flora; the polyethylene glycol electrolyte powder is used twice every day at an interval of 4 hours. The method has the advantages of short model construction time that is only 8 hours for a whole process, quickness, simplicity, feasibility,remarkable effect, no side effect and high repetition rate.

Description

technical field [0001] The invention relates to the technical field of intestinal flora research, in particular to a method for constructing an intestinal flora rodent model. Background technique [0002] Under normal physiological conditions, the human intestinal tract contains at least 1,000 different strains of bacteria, and the number of genes carried by these flora is more than 100 times that of the human body's own genes. The mutually beneficial dynamic balance between the host and the intestinal flora maintains the body's play a very important role in various functions. With the deepening of research, the relationship between intestinal flora and the body's disease and health has become increasingly clear, and it plays an irreplaceable role in maintaining the normal functions of the body's immune system, circulatory system, central nervous system and digestive system. Dysregulation may contribute to and / or exacerbate the development of various diseases, such as cance...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02
CPCA01K67/02
Inventor 许安刘颖程雷杨振汪童丁晗
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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