Method for constructing rodent model for intestinal flora
A technology for intestinal flora and rodents, applied in animal husbandry and other directions, can solve the problems of limited effect, limited effect of antibiotics, side effects, etc., and achieve the effect of short modeling time, remarkable effect and good repeatability
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[0032] (1) Establishment method of rodent model for intestinal flora research:
[0033] (1) 2-month-old C57BL / 6 mice were used as experimental animals, male, 10, body weight 30±2.0g, randomly divided into two groups: control group and model group (5 each); reared in a barrier environment, Feed the basic maintenance feed; the food and water required for the day are sterilized, and they can eat and drink freely;
[0034] Control group: Mice were fed with normal saline, twice a day, 1 mL / time / mouse, with an interval of 4 hours, and were fasted for 2 hours before feeding;
[0035] Model group: Mice were given polyethylene glycol electrolyte powder, twice a day, 1 mL / time / mouse, with an interval of 4 hours, and were fasted for 2 hours before feeding;
[0036] (2) Preparation of Polyethylene Glycol Electrolyte Powder: The preparation method of the used Polyethylene Glycol Electrolyte Powder is by potassium chloride 0.74g, sodium bicarbonate 1.68g, sodium chloride 1.46g, sodium sulf...
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