Method for inhibiting Phytophthora capsici by utilizing residue of Chinese herbal medicine for clearing away heat and toxic material
A heat-clearing and detoxifying technology for Phytophthora capsici, applied in the field of suppressing Phytophthora capsicum, can solve problems such as economic loss, waste of resources, and undeveloped system for the treatment of Chinese herbal medicine dregs, and achieve ecological balance, resource conservation, and less harm Effect
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Embodiment 1
[0011] Example 1: Preparation of alcohol extract of Chinese herbal medicine dregs with heat-clearing and detoxifying effects: The same dose of Chinese herbal medicine dregs with heat-clearing and detoxifying effects brought back from Taiyuan Central Hospital, the Chinese herbal medicines are specifically: Aster 12 grams, Winter Flower 12 grams , 12 grams of tangerine peel, 20 grams of silver flower, 20 grams of forsythia, 20 grams of Houttuynia cordata, 12 grams of Digupi, 12 grams of bletilla striata, 12 grams of Baiwei, 9 grams of almonds, 12 grams of turmeric, 12 grams of platycodon, Sichuan Achyranthes bidentata 15 grams, Chuan Bei 9 grams, licorice 6 grams.
[0012] The medicine dregs brought back were numbered 1 and 2, drained, and dried in the sun. Weighed 10 g of each medicine residue and put them into conical flasks, and added 100 mL of 80% ethanol to each conical flask , leached in a constant temperature oscillator for 2-3 days, filtered and centrifuged, and the supe...
Embodiment 2
[0013] Example 2: Plate experiments on the inhibition of Phytophthora capsici by the prepared heat-clearing and detoxifying Chinese herbal medicine dregs ethanol extract:
[0014] 1. Preparation of Phytophthora capsici suspension: On the ultra-clean workbench, elute the cells of Phytophthora capsicum on the inclined plane of the expanded cultured Phytophthora capsici test tube, and dilute the concentration of the bacterial suspension to about 10 6 CFU / mL. Store in the refrigerator for later use.
[0015] 2. Bacteriostatic experiment of the prepared alcoholic extract of Chinese herbal medicine dregs on Phytophthora capsici: Use a puncher to punch 4 holes evenly on the medium, and then use a pipette gun to inject the liquid into the holes. The first is 0.2 mL of the alcoholic extract of Chinese herbal medicine dregs, and the control is repeated three times. Then use a pipette gun to inject 0.2 mL of sterile water into the fourth hole as a blank control and label it. All cult...
Embodiment 3
[0018] Embodiment 3: the field control effect of the ethanol extract of Chinese herbal medicine dregs to Phytophthora capsici:
[0019] Field experiments were used to detect the control effect of Chinese herbal medicine dregs on Phytophthora capsicum. Transplant 3 healthy pepper seedlings at the 4-leaf stage. The settings were: ① Control 1: the soil was not inoculated with any microorganisms; ② Control 2: the soil was inoculated with Phytophthora capsici; ③ Treatment experiment: the soil was simultaneously added with the alcohol extract of Chinese herbal medicine No. 1 (concentration: 2 g / mL) and Phytophthora capsici . The inoculum amount of Phytophthora capsici is 500-800 sporangia in 1 g of air-dried soil; when the pepper is planted, 8-12 mL is evenly poured into it from the root. Each treatment had 6 plants, and the experiment was repeated three times. All controls and treatments were surveyed 7 and 14 days after transplanting, and the incidence was counted, and the inci...
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